Details of the PSS

General Information of Protein Scaffold of SBP (PSS) (ID: PS027)
PSS Name	DARPin
Synonyms	Designed ankyrin-repeat protein; Designed AR protein; Darpin; darpin
Diagrammatic Sketch
Highest Status	Preregistration
Class	Non-Antibody
Fold Type	Alpha-Helices + Loops
Thermal Denaturation TEMP	66-89℃
Sequence Length	67+33 * n
Molecular Weight	10-20 kDa

Parental Protein of This PSS

Parental Protein Name	Synonym		PDB ID	UniProt ID	Origin
Ankyrin repeat protein	AR protein		1MJ0	Q86SG2	Human

Synthetic Binding Protein (SBP) Featuring this PSS

SBP Name	Highest Status	Template	Expression System	Selection Method	Details	Ref
DARPin Abicipar pegol	Preregistration	N.A.	N.A.	N.A.	SBP Info	[1], [2], [3]
DARPin Ensovibep	Phase III	N.A.	N.A.	N.A.	SBP Info	[4], [5]
DARPin MP0250	Phase II	N.A.	Escherichia coli	Phage display and Ribosome display	SBP Info	[6], [7], [8]
DARPin MP0274	Phase I	N.A.	Escherichia coli	Phage display and Ribosome display	SBP Info	[6], [2], [9]
DARPin MP0310	Phase I	N.A.	N.A.	N.A.	SBP Info	[9], [5]
DARPin anti-AcrB 110819	Research	N3C	Bacteria	Ribosome display	SBP Info	[10]
DARPin anti-APH(3)IIIa AR_3b	Research	N3C	Escherichia coli	Ribosome display	SBP Info	[11], [12]
DARPin anti-APH(3)IIIa clone 3a	Research	N3C	Bacteria	Ribosome display	SBP Info	[10]
DARPin anti-Bo2C11 eBo01	Research	N2C	Escherichia coli XL1-Blue	Ribosome display	SBP Info	[13]
DARPin anti-Bo2C11 eBo38	Research	N2C	Escherichia coli XL1-Blue	Ribosome display	SBP Info	[13]
DARPin anti-Bo2C11 eBo89	Research	N2C	Escherichia coli XL1-Blue	Ribosome display	SBP Info	[13]
DARPin anti-Bo2C11 eBo90	Research	N2C	Escherichia coli XL1-Blue	Ribosome display	SBP Info	[13]
DARPin anti-CASP-2 F8	Research	N3C	Bacteria	Ribosome display	SBP Info	[10]
DARPin anti-Cathepsin-B clone 81	Research	AR module	Escherichia coli XL1-Blue	Ribosome display	SBP Info	[14], [15]
DARPin anti-Cathepsin-B clone 8h6	Research	AR module	Escherichia coli XL1-Blue	Ribosome display	SBP Info	[14], [15]
DARPin anti-CD4 clone 1.1	Research	AR module	Escherichia coli	Ribosome display	SBP Info	[16], [17]
DARPin anti-CD4 clone 2.1	Research	AR module	Escherichia coli	Ribosome display	SBP Info	[16], [17]
DARPin anti-CD4 clone 23.2	Research	AR module	Escherichia coli	Ribosome display	SBP Info	[16], [17]
DARPin anti-CD4 clone 25.2	Research	AR module	Escherichia coli	Ribosome display	SBP Info	[16], [17]
DARPin anti-CD4 clone 27.2	Research	AR module	Escherichia coli	Ribosome display	SBP Info	[16], [17]
DARPin anti-CD4 clone 29.2	Research	AR module	Escherichia coli	Ribosome display	SBP Info	[16], [17]
DARPin anti-CD4 clone 3.1	Research	AR module	Escherichia coli	Ribosome display	SBP Info	[16], [17]
DARPin anti-CD4 clone 4.1	Research	AR module	Escherichia coli	Ribosome display	SBP Info	[16], [17]
DARPin anti-CD4 clone 5.1	Research	AR module	Escherichia coli	Ribosome display	SBP Info	[16], [17]
DARPin anti-CD4 clone 55.2	Research	AR module	Escherichia coli	Ribosome display	SBP Info	[16], [17]
DARPin anti-CD4 clone 57.2	Research	AR module	Escherichia coli	Ribosome display	SBP Info	[16], [17]
DARPin anti-CD4 clone 6.1	Research	AR module	Escherichia coli	Ribosome display	SBP Info	[16], [17]
DARPin anti-EpCAM Ac1	Research	N3C	Escherichia coli	Ribosome display	SBP Info	[18], [19], [20]
DARPin anti-EpCAM Ac2	Research	N3C	Escherichia coli	Ribosome display	SBP Info	[18], [19], [20]
DARPin anti-EpCAM Ec2	Research	N3C	Escherichia coli	Ribosome display	SBP Info	[18], [19], [20]
DARPin anti-EpCAM Ec3	Research	N3C	Escherichia coli	Ribosome display	SBP Info	[18], [19], [20]
DARPin anti-EpCAM Ec4	Research	N3C	Escherichia coli	Ribosome display	SBP Info	[18], [19], [20]
DARPin anti-EpCAM Ec5	Research	N3C	Escherichia coli	Ribosome display	SBP Info	[18], [19], [20]
DARPin anti-EpoR A_R3	Research	AR module	Escherichia coli	Yeast display	SBP Info	[21]
DARPin anti-EpoR C_R3	Research	AR module	Escherichia coli	Yeast display	SBP Info	[21]
DARPin anti-EpoR E2	Research	N3C	Escherichia coli	Yeast display	SBP Info	[21]
DARPin anti-EpoR R12	Research	AR module	Escherichia coli	Yeast display	SBP Info	[21]
DARPin anti-ERBB1 E01	Research	AR module	Escherichia coli XL1-Blue	Phage display	SBP Info	[22], [23]
DARPin anti-ERBB1 E67	Research	N3C	Escherichia coli XL1-Blue	Phage display	SBP Info	[24], [25]
DARPin anti-ERBB1 E68	Research	N3C	Escherichia coli XL1-Blue	Phage display	SBP Info	[24], [25]
DARPin anti-ERBB2 6L1G	Research	AR module	Escherichia coli	Phage display and Ribosome display	SBP Info	[26], [27], [28]
DARPin anti-ERBB2 A1	Research	DARPin H10-2-G3	Escherichia coli M15	SNAP display	SBP Info	[29]
DARPin anti-ERBB2 C3	Research	DARPin H10-2-G3	Escherichia coli M15	SNAP display	SBP Info	[29]
DARPin anti-ERBB2 D4	Research	DARPin H10-2-G3	Escherichia coli M15	SNAP display	SBP Info	[29]
DARPin anti-ERBB2 F1	Research	DARPin H10-2-G3	Escherichia coli M15	SNAP display	SBP Info	[29]
DARPin anti-ERBB2 F3	Research	DARPin H10-2-G3	Escherichia coli M15	SNAP display	SBP Info	[29]
DARPin anti-ERBB2 G3-A	Research	N2C	Escherichia coli	Ribosome display	SBP Info	[30]
DARPin anti-ERBB2 G3-AVD	Research	N2C	Escherichia coli	Ribosome display	SBP Info	[30]
DARPin anti-ERBB2 G3-D	Research	N2C	Escherichia coli	Ribosome display	SBP Info	[30]
DARPin anti-ERBB2 G3-HAVD	Research	N2C	Escherichia coli	Ribosome display	SBP Info	[30]
DARPin anti-ERBB2 H10-2-A2	Research	N2C	Escherichia coli	Ribosome display	SBP Info	[30]
DARPin anti-ERBB2 H10-2-D11	Research	N2C	Escherichia coli	Ribosome display	SBP Info	[30]
DARPin anti-ERBB2 H10-2-D12	Research	N2C	Escherichia coli	Ribosome display	SBP Info	[30]
DARPin anti-ERBB2 H10-2-G3	Research	N2C	Escherichia coli	Ribosome display	SBP Info	[30], [31], [32]
DARPin anti-ERBB2 H10-2-G5	Research	N2C	Escherichia coli	Ribosome display	SBP Info	[30]
DARPin anti-ERK2 A443D	Research	DARPin E40	N.A.	N.A.	SBP Info	[33]
DARPin anti-ERK2 S380L	Research	DARPin E40	N.A.	N.A.	SBP Info	[33]
DARPin anti-Fc-epsilon-RIa clone B-A4-85	Research	AR module	Escherichia coli	Ribosome display	SBP Info	[34]
DARPin anti-Fc-epsilon-RIa clone C-A3-30	Research	AR module	Escherichia coli	Ribosome display	SBP Info	[34]
DARPin anti-GFP 3G124	Research	AR module	Escherichia coli XL1-Blue	Ribosome display	SBP Info	[35], [36]
DARPin anti-GFP 3G124nc	Research	AR module	Escherichia coli	Ribosome display	SBP Info	[37], [38], [36]
DARPin anti-GFP 3G168	Research	AR module	Escherichia coli XL1-Blue	Ribosome display	SBP Info	[35]
DARPin anti-GFP 3G61	Research	AR module	Escherichia coli	Ribosome display	SBP Info	[37], [35], [36]
DARPin anti-GFP 3G86.1	Research	AR module	Escherichia coli XL1-Blue	Ribosome display	SBP Info	[35]
DARPin anti-GFP 3G86.32	Research	AR module	Escherichia coli XL1-Blue	Ribosome display	SBP Info	[35], [39], [11]
DARPin anti-GluA4 2A10	Research	VV-N2C	Escherichia coli	Ribosome display	SBP Info	[40]
DARPin anti-GluA4 2A2	Research	VV-N2C	Escherichia coli	Ribosome display	SBP Info	[40]
DARPin anti-GluA4 2B7	Research	VV-N2C	Escherichia coli	Ribosome display	SBP Info	[40]
DARPin anti-GluA4 2G10	Research	VV-N2C	Escherichia coli	Ribosome display	SBP Info	[40]
DARPin anti-GluA4 2K19	Research	VV-N2C	Escherichia coli	Ribosome display	SBP Info	[40]
DARPin anti-GluA4 SA8	Research	S-N3C	Escherichia coli	Ribosome display	SBP Info	[40]
DARPin anti-GluA4 SB4	Research	S-N3C	Escherichia coli	Ribosome display	SBP Info	[40]
DARPin anti-GluA4 SC5	Research	S-N3C	Escherichia coli	Ribosome display	SBP Info	[40]
DARPin anti-GluA4 SD6	Research	S-N3C	Escherichia coli	Ribosome display	SBP Info	[40]
DARPin anti-GluA4 SD7	Research	S-N3C	Escherichia coli	Ribosome display	SBP Info	[40]
DARPin anti-GluA4 SD8	Research	S-N3C	Escherichia coli	Ribosome display	SBP Info	[40]
DARPin anti-GluA4/GluA2 SK14	Research	S-N3C	Escherichia coli	Ribosome display	SBP Info	[40]
DARPin anti-HDAC6-ZnF F10	Research	N.A.	Escherichia coli	ribosome display	SBP Info	[41]
DARPin anti-HER2/neu clone 9.16	Research	N3C	Escherichia coli BL21 (DE3)	Ribosome display; phage display	SBP Info	[42], [43]
DARPin anti-HER2/neu clone 9.26	Research	N3C	Escherichia coli BL21 (DE3)	Ribosome display; phage display	SBP Info	[42], [43], [44]
DARPin anti-HER2/neu clone 9.29	Research	N3C	Escherichia coli BL21 (DE3)	Ribosome display; phage display	SBP Info	[42], [43], [44]
DARPin anti-HER2/neu H14R	Research	N3C	Escherichia coli BL21 (DE3)	Ribosome display; phage display	SBP Info	[42], [43]
DARPin anti-IgE E07	Research	E3_53 T48N	N.A.	yeast display	SBP Info	[45]
DARPin anti-IgE E3_53	Research	N.A.	N.A.	yeast display	SBP Info	[45]
DARPin anti-KRas E3.5	Research	AR module	Human embryonic kidney?cells 293; HCT116 cells	Phage display	SBP Info	[46], [15]
DARPin anti-KRas K13	Research	AR module	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[46]
DARPin anti-KRas K19	Research	AR module	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[46]
DARPin anti-KRas K27	Research	AR module	Human embryonic kidney?cells 293; HCT116 cells	Phage display	SBP Info	[46], [47]
DARPin anti-KRas K55	Research	AR module	Human embryonic kidney?cells 293; HCT116 cells	Phage display	SBP Info	[46]
DARPin anti-MBP off7-M1	Research	DARPin off7	E. coli strains XL1-Blue	N.A.	SBP Info	[48]
DARPin anti-MBP off7-M2	Research	DARPin off7	E. coli strains XL1-Blue	N.A.	SBP Info	[48]
DARPin anti-MBP off7-M3	Research	DARPin off7	E. coli strains XL1-Blue	N.A.	SBP Info	[48]
DARPin anti-mCherry 2m151	Research	AR module	Escherichia coli XL1-Blue	Ribosome display	SBP Info	[35]
DARPin anti-mCherry 2m172	Research	AR module	Escherichia coli XL1-Blue	Ribosome display	SBP Info	[35]
DARPin anti-mCherry 2m22	Research	AR module	Escherichia coli XL1-Blue	Ribosome display	SBP Info	[35]
DARPin anti-mCherry 2m74	Research	AR module	Escherichia coli XL1-Blue	Ribosome display	SBP Info	[35]
DARPin anti-mCherry 3m160	Research	AR module	Escherichia coli XL1-Blue	Ribosome display	SBP Info	[35]
DARPin anti-PLK1 3H10	Research	N3C	Bacteria	Ribosome display	SBP Info	[10]
DARPin anti-Stx2a #3	Research	N.A.	E. coli BL21(DE3)	phage display	SBP Info	[49]
DARPin anti-Stx2a 10B	Research	N.A.	E. coli BL21(DE3)	phage display	SBP Info	[49]
DARPin anti-Stx2a SHT	Research	DARPin 10B	E. coli BL21(DE3)	phage display	SBP Info	[49]
DARPin anti-TcdB clone 1.2E	Research	AR module	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[50], [51]
DARPin anti-TcdB clone 1.4E	Research	AR module	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[50]
DARPin anti-TcdB clone U3	Research	AR module	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[50], [51]
DARPin anti-TFP1 1238_E11	Research	AR module	Escherichia coli XL1-Blue	Ribosome display	SBP Info	[37]
DARPin anti-TFP1 1238_G01	Research	AR module	Escherichia coli XL1-Blue	Ribosome display	SBP Info	[37]
DARPin anti-Tubulin A-C2	Research	AR module	Escherichia coli XL1-Blue	Ribosome display	SBP Info	[52]
DARPin anti-Tubulin TM-3	Research	AR module	Escherichia coli XL1-Blue	Ribosome display	SBP Info	[52]
DARPin anti-VEGF-A clone 1	Research	AR module	Escherichia coli XL1-Blue	Ribosome display	SBP Info	[53]
DARPin anti-VEGF-A clone 10	Research	AR module	Escherichia coli XL1-Blue	Ribosome display	SBP Info	[53]
DARPin anti-VEGF-A clone 2	Research	AR module	Escherichia coli XL1-Blue	Ribosome display	SBP Info	[53]
DARPin anti-VEGF-A clone 3	Research	AR module	Escherichia coli XL1-Blue	Ribosome display	SBP Info	[53]
DARPin anti-VEGF-A clone 4	Research	AR module	Escherichia coli XL1-Blue	Ribosome display	SBP Info	[53]
DARPin anti-VEGF-A clone 5	Research	AR module	Escherichia coli XL1-Blue	Ribosome display	SBP Info	[53]
DARPin anti-VEGF-A clone 6	Research	AR module	Escherichia coli XL1-Blue	Ribosome display	SBP Info	[53]
DARPin anti-VEGF-A clone 7	Research	AR module	Escherichia coli XL1-Blue	Ribosome display	SBP Info	[53]
DARPin anti-VEGF-A clone 8	Research	AR module	Escherichia coli XL1-Blue	Ribosome display	SBP Info	[53]
DARPin anti-VEGF-A clone 9	Research	AR module	Escherichia coli XL1-Blue	Ribosome display	SBP Info	[53]
DARPin anti-Bcl-2 008_H10	Research	N.A.	Escherichia coli	Ribosome display	SBP Info	[54]
DARPin anti-Bcl-2/BCL-XL/Bcl-W 008_C6	Research	N.A.	Escherichia coli	Ribosome display	SBP Info	[54]
DARPin anti-BPP clone 18	Research	N.A.	Escherichia coli XL1-Blue	Ribosome display	SBP Info	[55]
DARPin anti-BPP clone 19	Research	N.A.	Escherichia coli XL1-Blue	Ribosome display	SBP Info	[55]
DARPin anti-BPP clone 20	Research	N.A.	Escherichia coli XL1-Blue	Ribosome display	SBP Info	[55]
DARPin anti-CASP-1 D1.73	Research	N.A.	Escherichia coli	Ribosome display	SBP Info	[56]
DARPin anti-CASP-2 AR_F8	Research	N.A.	Escherichia coli	Ribosome display	SBP Info	[57]
DARPin anti-CASP-3 D3.4S76R	Research	N.A.	Hela cells	Ribosome display	SBP Info	[58]
DARPin anti-CASP-5 D5.15	Research	N.A.	Escherichia coli	Ribosome display	SBP Info	[56]
DARPin anti-CASP-6 D6.11	Research	N.A.	Escherichia coli	Ribosome display	SBP Info	[56]
DARPin anti-CASP-7 C7_16	Research	N.A.	Escherichia coli	Ribosome display	SBP Info	[59]
DARPin anti-CASP-7 D7.18	Research	N.A.	Escherichia coli	Ribosome display	SBP Info	[58]
DARPin anti-CASP-7 D7.43	Research	N.A.	Escherichia coli	Ribosome display	SBP Info	[58]
DARPin anti-CASP-8 D8.1	Research	N.A.	Escherichia coli	Ribosome display	SBP Info	[56]
DARPin anti-CASP-8 D8.4	Research	N.A.	Escherichia coli	Ribosome display	SBP Info	[56]
DARPin anti-CASP-9 D9.2	Research	N.A.	Escherichia coli	Ribosome display	SBP Info	[56]
DARPin anti-EpoR 8A9	Research	N.A.	Escherichia coli	Yeast display	SBP Info	[21]
DARPin anti-EpoR R11	Research	N.A.	Escherichia coli	Yeast display	SBP Info	[21]
DARPin anti-ERBB1 E69	Research	N.A.	Escherichia coli XL1-Blue	Phage display	SBP Info	[60], [24], [25]
DARPin anti-ERBB2 clone 28z	Research	AR module	T lymphocytes	N.A.	SBP Info	[61]
DARPin anti-ERBB2 D72C	Research	DARPin mut4	Escherichia coli	N.A.	SBP Info	[62]
DARPin anti-ERBB2 G3-E2_5	Research	N.A.	Escherichia coli XL1-Blue	Phage display and Ribosome display	SBP Info	[63]
DARPin anti-ERBB2 G3-G3	Research	N.A.	Escherichia coli XL1-Blue	Phage display and Ribosome display	SBP Info	[63]
DARPin anti-ERBB2 N69C	Research	DARPin mut4	Escherichia coli	N.A.	SBP Info	[62]
DARPin anti-ERK2 EpEM6	Research	N.A.	Escherichia coli	FLI-TRAP	SBP Info	[64]
DARPin anti-ERK2 EpEM7	Research	N.A.	Escherichia coli	FLI-TRAP	SBP Info	[64]
DARPin anti-ERK2 pE59	Research	N.A.	Escherichia coli	FLI-TRAP	SBP Info	[64]
DARPin anti-ERK2 pEM1	Research	N.A.	Escherichia coli	FLI-TRAP	SBP Info	[64]
DARPin anti-HER2/neu clone 9.01	Research	N.A.	Escherichia coli BL21 (DE3)	Ribosome display; phage display	SBP Info	[42]
DARPin anti-HIV-1 5m3_D12	Research	N.A.	Escherichia coli	Ribosome display	SBP Info	[65]
DARPin anti-IgE E53	Research	N.A.	Escherichia coli	Ribosome display	SBP Info	[34], [66]
DARPin anti-IgG-Fc I07	Research	N.A.	Lactococcus lactis cells	LysM-mediated lactococcus lactis surface display	SBP Info	[67], [68]
DARPin anti-IgG-Fc I19	Research	N.A.	Lactococcus lactis cells	LysM-mediated lactococcus lactis surface display	SBP Info	[67]
DARPin anti-IL-4 clone 44	Research	AR module	Escherichia coli BL21 (DE3)	N.A.	SBP Info	[69]
DARPin anti-LDB1 clone 3	Research	N.A.	Escherichia coli BL21 (DE3)	Ribosome display	SBP Info	[70]
DARPin anti-Listeriolysin-O D22	Research	N.A.	Escherichia coli BL21 (DE3)	Ribosome display	SBP Info	[71]
DARPin anti-MBP clone 16	Research	AR module	Escherichia coli BL21-CodonPlus (DE3)-RIL	N.A.	SBP Info	[72]
DARPin anti-MBP off7	Research	AR module	Escherichia coli BL21-CodonPlus (DE3)-RIL	N.A.	SBP Info	[72], [11], [73]
DARPin anti-MG FADA-3210	Research	N.A.	Escherichia coli XL1-Blue	Ribosome display; Yeast display	SBP Info	[74]
DARPin anti-MG FADA-C1	Research	N.A.	Escherichia coli XL1-Blue	Ribosome display; Yeast display	SBP Info	[74]
DARPin anti-MG FADA-D4	Research	N.A.	Escherichia coli XL1-Blue	Ribosome display; Yeast display	SBP Info	[74]
DARPin anti-NTSR1 D14	Research	N.A.	Escherichia coli XL1-Blue	Ribosome display	SBP Info	[75]
DARPin anti-NTSR1 P28	Research	N.A.	Escherichia coli XL1-Blue	Ribosome display	SBP Info	[75]
DARPin anti-NTSR1 P4	Research	N.A.	Escherichia coli XL1-Blue	Ribosome display	SBP Info	[75]
DARPin anti-Tubulin D1	Research	N.A.	Escherichia coli XL1-Blue	Ribosome display	SBP Info	[76], [52]
DARPin anti-VEGFR-2 D23b	Research	N.A.	Escherichia coli	Ribosome display	SBP Info	[77], [78]
DARPin anti-VEGFR-2 D4b	Research	N.A.	Escherichia coli	Ribosome display	SBP Info	[77], [78]
DARPin anti-VEGFR-2 D7b	Research	N.A.	Escherichia coli	Ribosome display	SBP Info	[77], [78]
DARPin anti-Abeta D23	Research	N.A.	Escherichia coli	N.A.	SBP Info	[79]
DARPin anti-EpCAM Ec1	Research	N.A.	Escherichia coli BLR	N.A.	SBP Info	[80], [81]
DARPin anti-ERBB2 9L1H	Research	AR module	N.A.	N.A.	SBP Info	[28]
DARPin anti-ERBB2 clone 9_29	Research	N.A.	Escherichia coli BL21 (DE3)	N.A.	SBP Info	[82], [83], [84]
DARPin anti-IgE E2_79	Research	N.A.	Escherichia coli	N.A.	SBP Info	[85]
DARPin anti-IL-13 clone 6G9	Research	N.A.	Escherichia coli BL21 (DE3)	N.A.	SBP Info	[86]
DARPin anti-ERBB1 PSC099	Research	N.A.	N.A.	N.A.	SBP Info	[87]
DARPin anti-ERBB1 PSC099-DGN549	Research	N.A.	N.A.	N.A.	SBP Info	[87]
DARPin anti-ERBB1 PSC106	Research	N.A.	N.A.	N.A.	SBP Info	[87]
DARPin anti-ERBB1 PSC106-DGN549	Research	N.A.	N.A.	N.A.	SBP Info	[87]
DARPin anti-ERBB1 PSC108-DGN549	Research	N.A.	N.A.	N.A.	SBP Info	[87]
DARPin anti-FAP/CD40 MP0317	Research	N.A.	N.A.	N.A.	SBP Info	[9], [5]
DARPin anti-Fc-gamma-RIIA/RIIB clone D11_D11	Research	N.A.	N.A.	N.A.	SBP Info	[34], [88]
DARPin anti-Fc-gamma-RIIA/RIIB clone D11_E53	Research	N.A.	N.A.	N.A.	SBP Info	[34], [88]
DARPin anti-GFP B6	Research	N.A.	N.A.	N.A.	SBP Info	[89]
DARPin anti-GFP G10	Research	N.A.	N.A.	N.A.	SBP Info	[89], [90]
DARPin anti-Listeriolysin-O D30	Research	N.A.	N.A.	N.A.	SBP Info	[71]
DARPin anti-Listeriolysin-O D6	Research	N.A.	N.A.	N.A.	SBP Info	[71]
DARPin anti-PD-1 DARPin-1	Research	N.A.	N.A.	N.A.	SBP Info	[91]
DARPin anti-PD-1 DARPin-2	Research	N.A.	N.A.	N.A.	SBP Info	[91]
DARPin anti-PDGF-B/VEGF-A MP0260	Research	N.A.	N.A.	N.A.	SBP Info	[5]
DARPin anti-SARS-CoV-2 MP0423	Research	N.A.	N.A.	N.A.	SBP Info	[4], [5]
DARPin anti-TNF-R2 clone 18	Research	N.A.	N.A.	N.A.	SBP Info	[92]

References

1	Double-Masked, Randomized, Phase 2 Evaluation of Abicipar Pegol (an Anti-VEGF DARPin Therapeutic) in Neovascular Age-Related Macular Degeneration. J Ocul Pharmacol Ther. 2018 Dec;34(10):700-709.
2	In vitro-engineered non-antibody protein therapeutics. Protein Cell. 2018 Jan;9(1):3-14.
3	A Mechanistic and Translational Pharmacokinetic-Pharmacodynamic Model of Abicipar Pegol and Vascular Endothelial Growth Factor Inhibition. J Pharmacol Exp Ther. 2020 May;373(2):184-192.
4	DARPins stack up as anti-COVID-19 agents. Nat Biotechnol. 2020 Dec;38(12):1369.
5	Molecular Partners. Product Development Pipeline. 2021.
6	DARPins: Promising Scaffolds for Theranostics. Acta Naturae. Oct-Dec 2019;11(4):42-53.
7	MP0250, a VEGF and HGF neutralizing DARPin ? molecule shows high anti-tumor efficacy in mouse xenograft and patient-derived tumor models. Oncotarget. 2017 Oct 11;8(58):98371-98383.
8	Beyond antibodies: ankyrins and DARPins. From basic research to drug approval. Curr Opin Pharmacol . 2020 Apr;51:93-101.
9	Beyond Antibodies: The DARPin ? Drug Platform. BioDrugs. 2020 Aug;34(4):423-433.
10	Chaperone-assisted crystallography with DARPins. Structure. 2008 Oct 8;16(10):1443-53.
11	Modular protein switches derived from antibody mimetic proteins. Protein Eng Des Sel. 2016 Feb;29(2):77-85.
12	Intracellular kinase inhibitors selected from combinatorial libraries of designed ankyrin repeat proteins. J Biol Chem. 2005 Jul 1;280(26):24715-22.
13	Designed ankyrin repeat proteins: a new approach to mimic complex antigens for diagnostic purposes?. PLoS One. 2013 Apr 23;8(4):e60688.
14	New Kid on the Block. Theranostics. 2017 Jul 30;7(11):2965-2967.
15	Non-invasive in vivo imaging of tumour-associated cathepsin B by a highly selective inhibitory DARPin. Theranostics. 2017 Jul 8;7(11):2806-2821.
16	Designed Ankyrin Repeat Protein (DARPin) to target chimeric antigen receptor (CAR)-redirected T cells towards CD4 + T cells to reduce the latent HIV + cell reservoir. Med Microbiol Immunol. 2020 Dec;209(6):681-691.
17	CD4-specific designed ankyrin repeat proteins are novel potent HIV entry inhibitors with unique characteristics. PLoS Pathog. 2008 Jul 25;4(7):e1000109.
18	Enhanced lysis by bispecific oncolytic measles viruses simultaneously using HER2/neu or EpCAM as target receptors. Mol Ther Oncolytics. 2016 Feb 24;3:16003.
19	DARPin-targeting of measles virus: unique bispecificity, effective oncolysis, and enhanced safety. Mol Ther. 2013 Apr;21(4):849-59.
20	DARPins recognizing the tumor-associated antigen EpCAM selected by phage and ribosome display and engineered for multivalency. J Mol Biol. 2011 Nov 4;413(4):826-43.
21	Topological control of cytokine receptor signaling induces differential effects in hematopoiesis. Science. 2019 May 24;364(6442):eaav7532.
22	Multispecific Targeting with Synthetic Ankyrin Repeat Motif Chimeric Antigen Receptors. Clin Cancer Res. 2019 Dec 15;25(24):7506-7516.
23	Bifunctional Reagents for Formylglycine Conjugation: Pitfalls and Breakthroughs. Chembiochem. 2020 Dec 11;21(24):3580-3593.
24	Recent progress in protein-protein interaction study for EGFR-targeted therapeutics. Expert Rev Proteomics. 2016 Sep;13(9):817-32.
25	Efficient selection of DARPins with sub-nanomolar affinities using SRP phage display. J Mol Biol. 2008 Oct 24;382(5):1211-27.
26	Systemic analysis of tyrosine kinase signaling reveals a common adaptive response program in a HER2-positive breast cancer. Sci Signal. 2019 Jan 22;12(565):eaau2875.
27	Designed ankyrin repeat proteins (DARPins) from research to therapy. Methods Enzymol. 2012;503:101-34.
28	Intermolecular biparatopic trapping of ErbB2 prevents compensatory activation of PI3K/AKT via RAS-p110 crosstalk. Nat Commun. 2016 Jun 3;7:11672.
29	Directed evolution of anti-HER2 DARPins by SNAP display reveals stability/function trade-offs in the selection process. Protein Eng Des Sel. 2015 Sep;28(9):269-79.
30	A designed ankyrin repeat protein evolved to picomolar affinity to Her2. J Mol Biol. 2007 Jun 15;369(4):1015-28.
31	Development of the designed ankyrin repeat protein (DARPin) G3 for HER2 molecular imaging. Eur J Nucl Med Mol Imaging. 2015 Feb;42(2):288-301.
32	Multifunctional superparamagnetic nanoparticles conjugated with fluorescein-labeled designed ankyrin repeat protein as an efficient HER2-targeted probe in breast cancer. Biomaterials. 2017 Dec;147:86-98.
33	Molecular Dynamics Simulations in Designing DARPins as Phosphorylation-Specific Protein Binders of ERK2. Molecules. 2021 Jul 27;26(15):4540.
34	Current Strategies to Inhibit High Affinity FcRI-Mediated Signaling for the Treatment of Allergic Disease. Front Immunol. 2019 Feb 7;10:175.
35	Protein interference applications in cellular and developmental biology using DARPins that recognize GFP and mCherry. Biol Open. 2014 Nov 21;3(12):1252-61.
36	Design and applications of a clamp for Green Fluorescent Protein with picomolar affinity. Sci Rep. 2017 Nov 24;7(1):16292.
37	DARPins recognizing mTFP1 as novel reagents for in vitro and in vivo protein manipulations. Biol Open. 2018 Oct 29;7(11):bio036749.
38	Tunable light and drug induced depletion of target proteins. Nat Commun. 2020 Jan 16;11(1):304.
39	Fusion of DARPin to Aldolase Enables Visualization of Small Protein by Cryo-EM. Structure. 2019 Jul 2;27(7):1148-1155.e3.
40	A Library-Based Screening Strategy for the Identification of DARPins as Ligands for Receptor-Targeted AAV and Lentiviral Vectors. Mol Ther Methods Clin Dev. 2018 Jul 6;10:128-143.
41	Disrupting the HDAC6-ubiquitin interaction impairs infection by influenza and Zika virus and cellular stress pathways. Cell Rep. 2022 Apr 26;39(4):110736.
42	DARPins: an efficient targeting domain for lentiviral vectors. Mol Ther. 2011 Apr;19(4):686-93.
43	A simple strategy for retargeting lentiviral vectors to desired cell types via a disulfide-bond-forming protein-peptide pair. Sci Rep. 2018 Jul 20;8(1):10990.
44	Retargeting Lentiviruses via SpyCatcher-SpyTag Chemistry for Gene Delivery into Specific Cell Types. mBio. 2017 Dec 12;8(6):e01860-17.
45	Structure-guided design of ultrapotent disruptive IgE inhibitors to rapidly terminate acute allergic reactions. J Allergy Clin Immunol. 2021 Oct;148(4):1049-1060.
46	KRAS-specific inhibition using a DARPin binding to a site in the allosteric lobe. Nat Commun. 2019 Jun 13;10(1):2607.
47	Structural and functional characterization of a DARPin which inhibits Ras nucleotide exchange. Nat Commun. 2017 Jul 14;8:16111.
48	Purification of MBP fusion proteins using engineered DARPin affinity matrix. Int J Biol Macromol. 2021 Sep 30;187:105-112.
49	A Multi-Specific DARPin Potently Neutralizes Shiga Toxin 2 via Simultaneous Modulation of Both Toxin Subunits. Bioengineering (Basel). 2022 Sep 27;9(10):511.
50	Selection and characterization of ultrahigh potency designed ankyrin repeat protein inhibitors of C. difficile toxin B. PLoS Biol. 2019 Jun 24;17(6):e3000311.
51	Designed Ankyrin Repeat Protein (DARPin) Neutralizers of TcdB from Clostridium difficile Ribotype 027. mSphere. 2019 Oct 2;4(5):e00596-19.
52	Destabilizing an interacting motif strengthens the association of a designed ankyrin repeat protein with tubulin. Sci Rep. 2016 Jul 6;6:28922.
53	Highly potent VEGF-A-antagonistic DARPins as anti-angiogenic agents for topical and intravitreal applications. Angiogenesis. 2013 Jan;16(1):101-11.
54	From DARPins to LoopDARPins: novel LoopDARPin design allows the selection of low picomolar binders in a single round of ribosome display. J Mol Biol. 2014 Feb 6;426(3):691-721.
55	Crystal structure and function of a DARPin neutralizing inhibitor of lactococcal phage TP901-1: comparison of DARPin and camelid VHH binding mode. J Biol Chem. 2009 Oct 30;284(44):30718-26.
56	Specific targeting of human caspases using designed ankyrin repeat proteins. Biol Chem. 2014 Oct;395(10):1243-52.
57	Inhibition of caspase-2 by a designed ankyrin repeat protein: specificity, structure, and inhibition mechanism. Structure. 2007 May;15(5):625-36.
58	Combined inhibition of caspase 3 and caspase 7 by two highly selective DARPins slows down cellular demise. Biochem J. 2014 Jul 15;461(2):279-90.
59	Design, construction, and characterization of a second-generation DARP in library with reduced hydrophobicity. Protein Sci. 2013 Sep;22(9):1239-57.
60	Bispecific designed ankyrin repeat proteins (DARPins) targeting epidermal growth factor receptor inhibit A431 cell proliferation and receptor recycling. J Biol Chem. 2011 Dec 2;286(48):41273-41285.
61	Designed ankyrin repeat proteins are effective targeting elements for chimeric antigen receptors. J Immunother Cancer. 2015 Dec 15;3:55.
62	A rapid, site-selective and efficient route to the dual modification of DARPins. Chem Commun (Camb). 2014 May 18;50(38):4898-900.
63	Efficient tumor targeting with high-affinity designed ankyrin repeat proteins: effects of affinity and molecular size. Cancer Res. 2010 Feb 15;70(4):1595-605.
64	A survival selection strategy for engineering synthetic binding proteins that specifically recognize post-translationally phosphorylated proteins. Nat Commun. 2019 Apr 23;10(1):1830.
65	Conformation-dependent recognition of HIV gp120 by designed ankyrin repeat proteins provides access to novel HIV entry inhibitors. J Virol. 2013 May;87(10):5868-81.
66	Inhibition of ongoing allergic reactions using a novel anti-IgE DARPin-Fc fusion protein. Allergy. 2011 Jul;66(7):961-8.
67	Improvement of LysM-mediated surface display of designed ankyrin repeat proteins (DARPins) in recombinant and nonrecombinant strains of Lactococcus lactis and Lactobacillus Species. Appl Environ Microbiol. 2015 Mar;81(6):2098-106.
68	Screening for New Surface Anchoring Domains for Lactococcus lactis. Front Microbiol. 2019 Aug 13;10:1879.
69	Induced conformational change in human IL-4 upon binding of a signal-neutralizing DARPin. Proteins. 2015 Jun;83(6):1191-7.
70	Rotational symmetry of the structured Chip/LDB-SSDP core module of the Wnt enhanceosome.Proc Natl Acad Sci USA. 2019 Oct 15;116(42):20977-20983.
71	Design of Protein Logic Gate System Operating on Lipid Membranes. ACS Synth Biol. 2020 Feb 21;9(2):316-328.
72	MBP-binding DARPins facilitate the crystallization of an MBP fusion protein. Acta Crystallogr F Struct Biol Commun. 2018 Sep 1;74(Pt 9):549-557.
73	Bioengineering of bacteria to assemble custom-made polyester affinity resins. Appl Environ Microbiol. 2015 Jan;81(1):282-91.
74	Generation of Fluorogen-Activating Designed Ankyrin Repeat Proteins (FADAs) as Versatile Sensor Tools. J Mol Biol. 2016 Mar 27;428(6):1272-1289.
75	Selection and characterization of DARPins specific for the neurotensin receptor 1. Protein Eng Des Sel. 2009 Jun;22(6):357-66.
76	Insight into microtubule disassembly by kinesin-13s from the structure of Kif2C bound to tubulin. Nat Commun. 2017 Jul 10;8(1):70.
77	Targeting extracellular domains D4 and D7 of vascular endothelial growth factor receptor 2 reveals allosteric receptor regulatory sites. Mol Cell Biol. 2012 Oct;32(19):3802-13.
78	High-affinity binders selected from designed ankyrin repeat protein libraries. Nat Biotechnol. 2004 May;22(5):575-82.
79	Amyloid- peptide-specific DARPins as a novel class of potential therapeutics for Alzheimer disease. J Biol Chem. 2014 Sep 26;289(39):27080-27089.
80	EpCAM-Binding DARPins for Targeted Photodynamic Therapy of Ovarian Cancer. Cancers (Basel). 2020 Jul 2;12(7):1762.
81	Radionuclide Molecular Imaging of EpCAM Expression in Triple-Negative Breast Cancer Using the Scaffold Protein DARPin Ec1. Molecules. 2020 Oct 14;25(20):4719.
82	On the prevention of kidney uptake of radiolabeled DARPins. EJNMMI Res. 2020 Feb 4;10(1):7.
83	Designed Ankyrin Repeat Proteins as Her2 Targeting Domains in Chimeric Antigen Receptor-Engineered T Cells. Hum Gene Ther. 2017 Sep;28(9):726-736.
84	Comparative Evaluation of Radioiodine and Technetium-Labeled DARPin 9_29 for Radionuclide Molecular Imaging of HER2 Expression in Malignant Tumors. Contrast Media Mol Imaging. 2018 Jun 6;2018:6930425.
85	Sensing and responding to allergic response cytokines through a genetically encoded circuit. Nat Commun. 2017 Oct 24;8(1):1101.
86	Conformational flexibility of an anti-IL-13 DARPin. Protein Eng Des Sel. 2017 Jan;30(1):31-37.
87	ImmunoGen. Product Development Pipeline. 2021.
88	A novel bispecific DARPin targeting FcRIIB and FcRI-bound IgE inhibits allergic responses. Allergy. 2017 Aug;72(8):1174-1183.
89	Structural analysis of biological targets by host:guest crystal lattice engineering. Sci Rep. 2019 Oct 23;9(1):15199.
90	Rigid fusions of designed helical repeat binding proteins efficiently protect a binding surface from crystal contacts. Sci Rep. 2019 Nov 7;9(1):16162.
91	Profound Functional Suppression of Tumor-Infiltrating T-Cells in Ovarian Cancer Patients Can Be Reversed Using PD-1-Blocking Antibodies or DARPin? Proteins. J Immunol Res. 2020 Aug 4;2020:7375947.
92	TNFR2 ligation in human T regulatory cells enhances IL2-induced cell proliferation through the non-canonical NF-B pathway. Sci Rep. 2018 Aug 13;8(1):12079.