Details of the SBP

General Information of Synthetic Binding Protein (SBP) (ID: SBP001483)
SBP Name
DARPin anti-ERBB1 E68
Synonyms
DARPin E68
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli XL1-Blue
Selection Method Phage display
Highest Status Research
Template Name N3C
Protein Scaffold Information of This SBP
Scaffold ID PS027
Scaffold Info
[1] , [2]
Scaffold Name DARPin
Scaffold Class Non-Antibody
Fold Type Alpha-Helices + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Epidermal growth factor receptor
BTS Info
Binder Cancers [ICD-11: 2D4Z] Kd: 0.7 nM Bielefeld University [1] , [2]
References
1 Recent progress in protein-protein interaction study for EGFR-targeted therapeutics. Expert Rev Proteomics. 2016 Sep;13(9):817-32.
2 Efficient selection of DARPins with sub-nanomolar affinities using SRP phage display. J Mol Biol. 2008 Oct 24;382(5):1211-27.