General Information of Synthetic Binding Protein (SBP) (ID: SBP003416)
SBP Name
DARPin anti-LDB1 clone 3
Synonyms
DARPin 3
Molecular Weight 15.9 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli BL21 (DE3)
Selection Method Ribosome display
Highest Status Research
PDB ID 6S9T
Sequence Length 151
SBP Sequence
>DARPin anti-LDB1 clone 3
GGGGSYPYDVPDYALEVLFQGPGSDLGKKLLEAATEGQDDEVRILMANGADVNAHDRLGS
TPLHLAAKMGHLEIVEVLLKTGADVNAEDTAGYTPLHLAAAWGHLEIVEVLLKHGADVNA
QDKFGKTPFDLAAIFGNEDIAEVLQKAAKLN
3D Structure
Experimentally Validated Structure
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Protein Scaffold Information of This SBP
Scaffold ID PS027
Scaffold Info
[1]
Scaffold Name DARPin
Scaffold Class Non-Antibody
Fold Type Alpha-Helices + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
LIM domain-binding protein 1
BTS Info
Binder Research tool N.A. Medical Research Council Laboratory of Molecular Biology [1]
References
1 Rotational symmetry of the structured Chip/LDB-SSDP core module of the Wnt enhanceosome.Proc Natl Acad Sci USA. 2019 Oct 15;116(42):20977-20983.