General Information of Synthetic Binding Protein (SBP) (ID: SBP000251)
SBP Name
DARPin anti-ERK2 EpEM6
Synonyms
DARPin EpEM6
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli
Selection Method FLI-TRAP
Highest Status Research
Protein Scaffold Information of This SBP
Scaffold ID PS027
Scaffold Info
[1]
Scaffold Name DARPin
Scaffold Class Non-Antibody
Fold Type Alpha-Helices + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Mitogen-activated protein kinase 1
BTS Info
Binder Tools for recognizing post-translationally phosphorylated proteins Kd: 633 nM Cornell University [1]
Mitogen-activated protein kinase 1
BTS Info
Binder Tools for recognizing post-translationally phosphorylated proteins Kd: 784 nM Cornell University [1]
References
1 A survival selection strategy for engineering synthetic binding proteins that specifically recognize post-translationally phosphorylated proteins. Nat Commun. 2019 Apr 23;10(1):1830.