General Information of Synthetic Binding Protein (SBP) (ID: SBP001486)
SBP Name
DARPin anti-Bo2C11 eBo38
Synonyms
DARPin eBo38
Molecular Weight 13.4 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli XL1-Blue
Selection Method Ribosome display
Highest Status Research
Sequence Length 126
SBP Sequence
>DARPin anti-Bo2C11 eBo38
GSDLGKKLLEAARAGQDDEVRILMANGADVNATGFMGTTPLHLAADNGHLEIVEVLLKHG
ADVNAADKYGTTPLHLAADYGHLEIVEVLLKYGADVNAQDKFGKTAFDISIDNGNEDLAE
ILQKLN
3D Structure
Computationally Modelled Structure
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Template Name N2C
Protein Scaffold Information of This SBP
Scaffold ID PS027
Scaffold Info
[1]
Scaffold Name DARPin
Scaffold Class Non-Antibody
Fold Type Alpha-Helices + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Bo2C11
BTS Info
Inhibitor Tools for mimicing complex antigens for diagnostic purposes Kd: 25.3 nM University of Bern [1]
References
1 Designed ankyrin repeat proteins: a new approach to mimic complex antigens for diagnostic purposes?. PLoS One. 2013 Apr 23;8(4):e60688.