Details of the SBP

General Information of Synthetic Binding Protein (SBP) (ID: SBP001493)
SBP Name
DARPin anti-Tubulin D1
Synonyms
DARPin D1
Molecular Weight 16.8 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli XL1-Blue
Selection Method Ribosome display
Highest Status Research
PDB ID 4DUI
Sequence Length 159
SBP Sequence
>DARPin anti-Tubulin D1
GSDLGKKLLEAARAGQDDEVRILMANGADVNATDASGLTPLHLAATYGHLEIVEVLLKHG
ADVNAIDIMGSTPLHLAALIGHLEIVEVLLKHGADVNAVDTWGDTPLHLAAIMGHLEIVE
VLLKHGADVNAQDKFGKTAFDISIDNGNEDLAEILQKLN
3D Structure
Experimentally Validated Structure
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Protein Scaffold Information of This SBP
Scaffold ID PS027
Scaffold Info
[1] , [2]
Scaffold Name DARPin
Scaffold Class Non-Antibody
Fold Type Alpha-Helices + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Tubulin
BTS Info
Binder Tools for microtubule disassembly Kd: 127 nM Tongji University; University of Sacre [1] , [2]
References
1 Insight into microtubule disassembly by kinesin-13s from the structure of Kif2C bound to tubulin. Nat Commun. 2017 Jul 10;8(1):70.
2 Destabilizing an interacting motif strengthens the association of a designed ankyrin repeat protein with tubulin. Sci Rep. 2016 Jul 6;6:28922.