Details of the SBP | SYNBIP

General Information of Synthetic Binding Protein (SBP) (ID: SBP003036)
SBP Name	DARPin anti-CD4 clone 23.2
Synonyms	DARPin 23.2
Molecular Weight	13.4 kDa
Design Method	Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System	Escherichia coli
Selection Method	Ribosome display
Highest Status	Research
Sequence Length	126
Template Name	AR module

Protein Scaffold Information of This SBP
Scaffold ID	PS027 Scaffold Info					[1] , [2]
Scaffold Name	DARPin
Scaffold Class	Non-Antibody
Fold Type	Alpha-Helices + Loops

Binding Target(s) of This SBP (BTS)

BTS Name	Details	Mechanism	Application	Affinity	Research Organization	Ref
T-cell surface glycoprotein CD4	BTS Info	Inhibitor	Human immunodeficiency virus infection (HIV infection) [ICD-11: 1C62.Z]	Kd: 0.26 nM	Paul-Ehrlich-Institut	[1] , [2]

References

1	Designed Ankyrin Repeat Protein (DARPin) to target chimeric antigen receptor (CAR)-redirected T cells towards CD4 + T cells to reduce the latent HIV + cell reservoir. Med Microbiol Immunol. 2020 Dec;209(6):681-691.
2	CD4-specific designed ankyrin repeat proteins are novel potent HIV entry inhibitors with unique characteristics. PLoS Pathog. 2008 Jul 25;4(7):e1000109.