Details of the SBP

General Information of Synthetic Binding Protein (SBP) (ID: SBP001475)
SBP Name
DARPin anti-VEGFR-2 D4b
Synonyms
DARPin D4b
Molecular Weight 17.1 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli
Selection Method Ribosome display
Highest Status Research
PDB ID 5OYJ
Sequence Length 159
3D Structure
Experimentally Validated Structure
Click to Save PDB File
Protein Scaffold Information of This SBP
Scaffold ID PS027
Scaffold Info
[1] , [2]
Scaffold Name DARPin
Scaffold Class Non-Antibody
Fold Type Alpha-Helices + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Vascular endothelial growth factor receptor 2
BTS Info
Inhibitor Diagnostic reagent Kd: 0.19 nM Paul Scherrer Institute [1] , [2]
References
1 Targeting extracellular domains D4 and D7 of vascular endothelial growth factor receptor 2 reveals allosteric receptor regulatory sites. Mol Cell Biol. 2012 Oct;32(19):3802-13.
2 High-affinity binders selected from designed ankyrin repeat protein libraries. Nat Biotechnol. 2004 May;22(5):575-82.