General Information of Synthetic Binding Protein (SBP) (ID: SBP001411)
SBP Name
DARPin anti-TFP1 1238_E11
Synonyms
DARPin 1238_E11
Molecular Weight 18.2 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli XL1-Blue
Selection Method Ribosome display
Highest Status Research
PDB ID 6FP9
Sequence Length 168
SBP Sequence
>DARPin anti-TFP1 1238_E11
DLGKKLLEAARAGQDDEVRILMANGADVNATDWVGMTPLHLAAWKGHLEIVEVLLKTGAD
VNAHDVFGTTPLHLAAHRGHLEIVEVLLKAGADVNAQDMVGKTPLHLAAYYGHLEIVEVL
LKHGADVNAQDKFGKTPFDLAIDNGNEDIAEVLQKAAKLNDYKDDDDK
3D Structure
Experimentally Validated Structure
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Template Name AR module
Protein Scaffold Information of This SBP
Scaffold ID PS027
Scaffold Info
[1]
Scaffold Name DARPin
Scaffold Class Non-Antibody
Fold Type Alpha-Helices + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Teal fluorescent protein 1
BTS Info
Binder Tools as novel reagents for in vitro and in vivo protein manipulations Kd: 3 nM University of Basel [1]
References
1 DARPins recognizing mTFP1 as novel reagents for in vitro and in vivo protein manipulations. Biol Open. 2018 Oct 29;7(11):bio036749.