General Information of Synthetic Binding Protein (SBP) (ID: SBP001499)
SBP Name
DARPin anti-Tubulin A-C2
Synonyms
DARPin A-C2
Molecular Weight 16.7 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli XL1-Blue
Selection Method Ribosome display
Highest Status Research
PDB ID 5EYP
Sequence Length 159
SBP Sequence
>DARPin anti-Tubulin A-C2
GSDLGKKLLEAARAGQDDEVRVLMANGADVNATDASGLTPLHLAATYGHLEIVEVLLKHG
ADVSASDLMGSTPLHLAALIGHLEIVEVLLKHGADVNAVDTWGDTPLRLAAVMGHLKIVE
ALLKHGADVNAQDKFGKTAYDTSIDNGSEDLAEILQKLN
3D Structure
Experimentally Validated Structure
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Template Name AR module
Protein Scaffold Information of This SBP
Scaffold ID PS027
Scaffold Info
[1]
Scaffold Name DARPin
Scaffold Class Non-Antibody
Fold Type Alpha-Helices + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Tubulin
BTS Info
Binder Tools as an improved affinity for tubulin Kd: 0.82 nM University of Sacre [1]
References
1 Destabilizing an interacting motif strengthens the association of a designed ankyrin repeat protein with tubulin. Sci Rep. 2016 Jul 6;6:28922.