Details of the SBP | SYNBIP

General Information of Synthetic Binding Protein (SBP) (ID: SBP001437)
SBP Name	DARPin anti-ERBB1 E69
Synonyms	DARPin E69
Design Method	Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System	Escherichia coli XL1-Blue
Selection Method	Phage display
Highest Status	Research

Protein Scaffold Information of This SBP
Scaffold ID	PS027 Scaffold Info					[1] , [2] , [3]
Scaffold Name	DARPin
Scaffold Class	Non-Antibody
Fold Type	Alpha-Helices + Loops

Binding Target(s) of This SBP (BTS)

BTS Name	Details	Mechanism	Application	Affinity	Research Organization	Ref
Epidermal growth factor receptor	BTS Info	Binder	Cancers [ICD-11: 2D4Z]	N.A.	University of Zurich; Bielefeld University	[1] , [2] , [3]

References

1	Bispecific designed ankyrin repeat proteins (DARPins) targeting epidermal growth factor receptor inhibit A431 cell proliferation and receptor recycling. J Biol Chem. 2011 Dec 2;286(48):41273-41285.
2	Recent progress in protein-protein interaction study for EGFR-targeted therapeutics. Expert Rev Proteomics. 2016 Sep;13(9):817-32.
3	Efficient selection of DARPins with sub-nanomolar affinities using SRP phage display. J Mol Biol. 2008 Oct 24;382(5):1211-27.