General Information of Synthetic Binding Protein (SBP) (ID: SBP003242)
SBP Name
DARPin anti-mCherry 2m172
Synonyms
DARPin 2m172
Molecular Weight 9.9 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli XL1-Blue
Selection Method Ribosome display
Highest Status Research
Sequence Length 93
SBP Sequence
>DARPin anti-mCherry 2m172
GSDLGKKLLEAARAGQDDEVRILMANGADVNADDITGITPLHLAAFTGHLEIVEVLLKNG
ADVNAQDKFGKTAFDISIDNGNEDLAEILQKLN
3D Structure
Computationally Modelled Structure
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Template Name AR module
Protein Scaffold Information of This SBP
Scaffold ID PS027
Scaffold Info
[1]
Scaffold Name DARPin
Scaffold Class Non-Antibody
Fold Type Alpha-Helices + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
MCHERRY
BTS Info
Binder Research tool N.A. University of Basel [1]
References
1 Protein interference applications in cellular and developmental biology using DARPins that recognize GFP and mCherry. Biol Open. 2014 Nov 21;3(12):1252-61.