Details of the SBP

General Information of Synthetic Binding Protein (SBP) (ID: SBP001432)
SBP Name
DARPin anti-HER2/neu clone 9.26
Synonyms
DARPin 9.26
Molecular Weight 17.8 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli BL21 (DE3)
Selection Method Ribosome display; phage display
Highest Status Research
Sequence Length 167
SBP Sequence
>DARPin anti-HER2/neu clone 9.26
SSGLVPRGSHMGSDLGKKLLEAARAGQDDEVRILMANGADVNAKDFYGITPLHLAAAYGH
LEIVEVLLKHGADVNAHDWNGWTPLHLAAKYGHLEIVEVLLKHGADVNAIDNAGKTPLHL
AAAHGHLEIVEVLLKYGADVNAQDKFGKTAFDISIDNGNEDLAEILQ
3D Structure
Computationally Modelled Structure
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Template Name N3C
Protein Scaffold Information of This SBP
Scaffold ID PS027
Scaffold Info
[1] , [2] , [3]
Scaffold Name DARPin
Scaffold Class Non-Antibody
Fold Type Alpha-Helices + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Receptor tyrosine-protein kinase erbB-2
BTS Info
Binder Cancers [ICD-11: 2D4Z] Kd: 1.4 nM Paul-Ehrlich-Institut; University of Zurich [1] , [2] , [3]
References
1 DARPins: an efficient targeting domain for lentiviral vectors. Mol Ther. 2011 Apr;19(4):686-93.
2 A simple strategy for retargeting lentiviral vectors to desired cell types via a disulfide-bond-forming protein-peptide pair. Sci Rep. 2018 Jul 20;8(1):10990.
3 Retargeting Lentiviruses via SpyCatcher-SpyTag Chemistry for Gene Delivery into Specific Cell Types. mBio. 2017 Dec 12;8(6):e01860-17.