General Information of Synthetic Binding Protein (SBP) (ID: SBP001424)
SBP Name
DARPin anti-KRas K27
Synonyms
DARPin K27
Molecular Weight 17.9 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Human embryonic kidney?cells 293; HCT116 cells
Selection Method Phage display
Highest Status Research
PDB ID 5O2S
Sequence Length 166
SBP Sequence
>DARPin anti-KRas K27
SSGHIEGRHMDLGKKLLEAARAGQDDEVRILMANGADVNAHDTFGFTPLHLAALYGHLEI
VEVLLKNGADVNADDSYGRTPLHLAAMRGHLEIVEVLLKYGADVNAADEEGRTPLHLAAK
RGHLEIVEVLLKNGADVNAQDKFGKTAFDISIDNGNEDLAEILQKL
3D Structure
Experimentally Validated Structure
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Template Name AR module
Protein Scaffold Information of This SBP
Scaffold ID PS027
Scaffold Info
[1] , [2]
Scaffold Name DARPin
Scaffold Class Non-Antibody
Fold Type Alpha-Helices + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
GTPase KRas
BTS Info
Inhibitor Cancers [ICD-11: 2D4Z] Kd: 3.9 nM University of Oxford; MedImmune [1] , [2]
References
1 KRAS-specific inhibition using a DARPin binding to a site in the allosteric lobe. Nat Commun. 2019 Jun 13;10(1):2607.
2 Structural and functional characterization of a DARPin which inhibits Ras nucleotide exchange. Nat Commun. 2017 Jul 14;8:16111.