General Information of Synthetic Binding Protein (SBP) (ID: SBP003615)
SBP Name
DARPin anti-MBP off7-M2
Synonyms
DARPin off7 M2
Molecular Weight 17.1 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System E. coli strains XL1-Blue
Highest Status Research
Sequence Length 159
SBP Sequence
>DARPin anti-MBP off7-M2
GSDLGRKLLEAARAGQDDEVRILMANGADVNAADNTGTTPLHLAAYSGHLEIVEVLLKHG
ADVDASDVFGYTPLHLAAYWGHLEIVEVLLKNGADVNAMDSDGMTPLHLAAKWGYLEIVE
VLLKHGADVNAQDRFGRTAFDISIDNGNEDLAEILQKLN
3D Structure
Computationally Modelled Structure
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Template Name DARPin off7
Protein Scaffold Information of This SBP
Scaffold ID PS027
Scaffold Info
[1]
Scaffold Name DARPin
Scaffold Class Non-Antibody
Fold Type Alpha-Helices + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Maltose/maltodextrin-binding periplasmic protein
BTS Info
binder Tools for purificating of MBP fusion proteins Kd: 114 nM ?afrik University [1]
References
1 Purification of MBP fusion proteins using engineered DARPin affinity matrix. Int J Biol Macromol. 2021 Sep 30;187:105-112.