General Information of Synthetic Binding Protein (SBP) (ID: SBP003614)
SBP Name
DARPin anti-MBP off7-M1
Synonyms
DARPin off7 M1
Molecular Weight 17.1 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System E. coli strains XL1-Blue
Highest Status Research
Sequence Length 159
SBP Sequence
>DARPin anti-MBP off7-M1
GSDLGRRLLEAARAGQDDEVRILMANGADVNAADNTGTTPLHLAAYSGHLEIVEVLLKHG
ADVDASDVFGYTPLHLAAYWGHLEIVEVLLKNGADVNAMDSDGMTPLHLAAKWGYLEIVE
VLLKHGADVNAQDKFGKTAFDISIDNGNEDLAEILQKLN
3D Structure
Computationally Modelled Structure
Click to Save PDB File
Template Name DARPin off7
Protein Scaffold Information of This SBP
Scaffold ID PS027
Scaffold Info
[1]
Scaffold Name DARPin
Scaffold Class Non-Antibody
Fold Type Alpha-Helices + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Maltose/maltodextrin-binding periplasmic protein
BTS Info
binder Tools for purificating of MBP fusion proteins Kd: 65 nM ?afrik University [1]
References
1 Purification of MBP fusion proteins using engineered DARPin affinity matrix. Int J Biol Macromol. 2021 Sep 30;187:105-112.