Details of the PSS

General Information of Protein Scaffold of SBP (PSS) (ID: PS051)
PSS Name	Peptide aptamer
Diagrammatic Sketch
Highest Status	Research
Class	Non-Antibody
Fold Type	Alpha-Helices + Beta-Sheets + Loops
Sequence Length	108-169
Molecular Weight	11-22 kDa

Parental Protein of This PSS

Parental Protein Name	Synonym	PDB ID	UniProt ID	Origin
Green fluorescent protein	GFP	1GFL	P42212	Aequorea victoria; Synthetic construct
Protease inhibitor eglin C	Eglin C	1EGP	A0A291RBX5	Poecilobdella manillensis
Staphylococcal nuclease	SN	1JOK	Q7KZF4	Human; Synthetic construct
Stefin A triple mutant	STM	N.A.	N.A.	Synthetic construct
Thioredoxin A	TrxA	6H1Y	P0AA25	Escherichia coli; Synthetic construct
Transcription factor Sp1	Sp1	1VA2	P08047 ; O89090	Human; Mouse

Synthetic Binding Protein (SBP) Featuring this PSS

SBP Name	Highest Status	Template	Expression System	Selection Method	Details	Ref
Peptide aptamer anti-ArfA clone 2	Research	Staphylococcus aureus nuclease	Aeromonas veronii C4	Bacterial two-hybrid system	SBP Info	[1]
Peptide aptamer anti-CaM SNP-D4	Research	Staphylococcus aureus nuclease	Escherichia coli XL1-Blue	Bacterial two-hybrid system	SBP Info	[2]
Peptide aptamer anti-ERBB1 KDI1	Research	Thioredoxin (TrxA)	Escherichia coli BL21 (DE3)	Yeast two-hybrid screen	SBP Info	[3], [4]
Peptide aptamer anti-HBV clone 34	Research	Thioredoxin (TrxA)	Huh-7 cells	Yeast two-hybrid screen	SBP Info	[5], [4]
Peptide aptamer anti-Id clone 10	Research	Thioredoxin (TrxA)	Bacteria	Yeast two-hybrid screen	SBP Info	[6], [4]
Peptide aptamer anti-Id clone 13	Research	Thioredoxin (TrxA)	Bacteria	Yeast two-hybrid screen	SBP Info	[6], [4]
Peptide aptamer anti-Id clone 17	Research	Thioredoxin (TrxA)	Bacteria	Yeast two-hybrid screen	SBP Info	[6], [4]
Peptide aptamer anti-Id clone 5	Research	Thioredoxin (TrxA)	Bacteria	Yeast two-hybrid screen	SBP Info	[6], [4]
Peptide aptamer anti-Id clone 7	Research	Thioredoxin (TrxA)	Bacteria	Yeast two-hybrid screen	SBP Info	[6], [4]
Peptide aptamer anti-LMO2 PA207	Research	Thioredoxin (TrxA)	Escherichia coli C41 (DE3)	Yeast two-hybrid screen	SBP Info	[7], [4]
Peptide aptamer anti-PrP clone 8	Research	Thioredoxin (TrxA)	Escherichia coli	Yeast two-hybrid screen	SBP Info	[8]
Peptide aptamer anti-PrP clone 8-46K	Research	Thioredoxin (TrxA)	Escherichia coli	Yeast two-hybrid screen	SBP Info	[8]
Peptide aptamer anti-PrP clone 8-46Q	Research	Thioredoxin (TrxA)	Escherichia coli	Yeast two-hybrid screen	SBP Info	[8]
Peptide aptamer anti-PrP clone 8-47H	Research	Thioredoxin (TrxA)	Escherichia coli	Yeast two-hybrid screen	SBP Info	[8]
Peptide aptamer anti-Rep A22	Research	Thioredoxin (TrxA)	T1 transgenic lines	Yeast two-hybrid screen	SBP Info	[9]
Peptide aptamer anti-Rep A64	Research	Thioredoxin (TrxA)	T1 transgenic lines	Yeast two-hybrid screen	SBP Info	[9]
Peptide aptamer anti-SmpB clone 1	Research	Staphylococcus aureus nuclease	Escherichia coli BL21 (DE3)	Bacterial two-hybrid system	SBP Info	[10]
Peptide aptamer anti-Ti minTBP-1	Research	N.A.	N.A.	Phage display	SBP Info	[11]
Peptide aptamer anti-VACV Apt72	Research	Thioredoxin (TrxA)	Human embryonic kidney?cells 293	Yeast two-hybrid screen	SBP Info	[12], [4]
Peptide aptamer anti-HBV C1-1	Research	N.A.	Mammalian cells	Yeast two-hybrid screen	SBP Info	[13], [4]
Peptide aptamer anti-HPV16 16E730	Research	N.A.	Huh-7 cells	Yeast two-hybrid screen	SBP Info	[5]
Peptide aptamer anti-MT1-MMP Swiggle	Research	Thioredoxin (TrxA)	Human cells	N.A.	SBP Info	[14], [4]
Peptide aptamer anti-PvCesA2 NoPv1	Research	N.A.	Escherichia coli DH5 (alpha)	Yeast two-hybrid screen	SBP Info	[15]
Peptide aptamer anti-SARS-CoV-2	Research	Thioredoxin (TrxA)	Escherichia coli	N.A.	SBP Info	[16]
Peptide aptamer anti-ThyA pBMT101	Research	N.A.	Escherichia coli	Yeast two-hybrid screen	SBP Info	[17], [4]
Peptide aptamer anti-ThyA pBMT101A	Research	N.A.	Escherichia coli	Yeast two-hybrid screen	SBP Info	[17], [4]
Peptide aptamer anti-ThyA pBMT101Q	Research	N.A.	Escherichia coli	Yeast two-hybrid screen	SBP Info	[17], [4]
Peptide aptamer anti-ThyA pBMT101S	Research	N.A.	Escherichia coli	Yeast two-hybrid screen	SBP Info	[17], [4]
Peptide aptamer anti-TiO2 Dps-ST1	Research	N.A.	Escherichia coli	Phage display	SBP Info	[18]
Peptide aptamer anti-CE Pi-101	Research	N.A.	N.A.	cDNA display	SBP Info	[19], [4]
Peptide aptamer anti-HCV 6B48	Research	N.A.	N.A.	mRNA display	SBP Info	[20], [4]
Peptide aptamer anti-HCV 6B48C	Research	N.A.	N.A.	mRNA display	SBP Info	[20], [4]
Peptide aptamer anti-HCV 6B4C	Research	N.A.	N.A.	mRNA display	SBP Info	[20], [4]
Peptide aptamer anti-HCV linear 6B4	Research	N.A.	N.A.	mRNA display	SBP Info	[20], [4]
Peptide aptamer anti-HPV16 E61-1	Research	Thioredoxin (TrxA)	N.A.	N.A.	SBP Info	[21]
Peptide aptamer anti-REN clone I-7	Research	N.A.	N.A.	cDNA display	SBP Info	[22]
Peptide aptamer anti-REN clone III-13	Research	N.A.	N.A.	cDNA display	SBP Info	[22]
Peptide aptamer anti-REN clone III-22	Research	N.A.	N.A.	cDNA display	SBP Info	[22]
Peptide aptamer anti-SOX2 P42	Research	Thioredoxin (TrxA)	N.A.	N.A.	SBP Info	[23]
Peptide aptamer anti-AK P3	Research	N.A.	N.A.	N.A.	SBP Info	[24]
Peptide aptamer anti-Bcl-6 Apt48	Research	N.A.	N.A.	N.A.	SBP Info	[25], [4]
Peptide aptamer anti-CDK1 Pep8	Research	N.A.	N.A.	N.A.	SBP Info	[26]
Peptide aptamer anti-CDK2 Pep4	Research	N.A.	N.A.	N.A.	SBP Info	[26]
Peptide aptamer anti-CKII-beta P1	Research	N.A.	N.A.	N.A.	SBP Info	[4]
Peptide aptamer anti-ERBB2 AII-7	Research	N.A.	N.A.	N.A.	SBP Info	[27], [4]
Peptide aptamer anti-Hsp70 A8/17	Research	N.A.	N.A.	N.A.	SBP Info	[28], [4]
Peptide aptamer anti-PSMA DUP-1	Research	N.A.	N.A.	N.A.	SBP Info	[29], [30]
Peptide aptamer anti-Ras Pep141	Research	N.A.	N.A.	N.A.	SBP Info	[31], [4]
Peptide aptamer anti-Ras Pep2	Research	N.A.	N.A.	N.A.	SBP Info	[31], [4]
Peptide aptamer anti-Ras Pep22	Research	N.A.	N.A.	N.A.	SBP Info	[31], [4]
Peptide aptamer anti-Smad Trx-CBP	Research	N.A.	N.A.	N.A.	SBP Info	[32], [4]
Peptide aptamer anti-Smad Trx-Lef1	Research	N.A.	N.A.	N.A.	SBP Info	[32], [4]
Peptide aptamer anti-Smad Trx-xFoxHlb	Research	N.A.	N.A.	N.A.	SBP Info	[32], [4]
Peptide aptamer anti-Smad4 TrxLef1D	Research	N.A.	N.A.	N.A.	SBP Info	[33], [4]
Peptide aptamer anti-STAT3 rS3-PA	Research	N.A.	N.A.	N.A.	SBP Info	[34]
Peptide aptamer anti-STAT5 S5-DBD-PA	Research	N.A.	N.A.	N.A.	SBP Info	[35]
Peptide aptamer anti-Tgat-GEF TRIP(E32G)	Research	N.A.	N.A.	N.A.	SBP Info	[36], [36]
Peptide aptamer anti-TSC2 Phi1	Research	N.A.	N.A.	N.A.	SBP Info	[37], [4]

References

1	Genetic Selection of Peptide Aptamers That Interact and Inhibit Both Small Protein B and Alternative Ribosome-Rescue Factor A of Aeromonas veronii C4. Front Microbiol. 2016 Aug 18;7:1228.
2	Engineering a peptide aptamer to target calmodulin for the inhibition of Magnaporthe oryzae. Fungal Biol. 2019 Jul;123(7):489-496.
3	Sequence-specific peptide aptamers, interacting with the intracellular domain of the epidermal growth factor receptor, interfere with Stat3 activation and inhibit the growth of tumor cells. J Biol Chem. 2003 Sep 26;278(39):37610-21.
4	Peptide aptamers with biological and therapeutic applications. Curr Med Chem. 2011;18(27):4215-22.
5	Peptide aptamer-mediated inhibition of target proteins by sequestration into aggresomes. J Biol Chem. 2006 Jul 28;281(30):21345-21352.
6	Targeting Id1 and Id3 by a specific peptide aptamer induces E-box promoter activity, cell cycle arrest, and apoptosis in breast cancer cells. Breast Cancer Res Treat. 2010 Dec;124(3):623-33
7	Targeting LMO2 with a peptide aptamer establishes a necessary function in overt T-cell neoplasia. Cancer Res. 2009 Jun 1;69(11):4784-90.
8	Interaction of Peptide Aptamers with Prion Protein Central Domain Promotes -Cleavage of PrP C. Mol Neurobiol. 2018 Oct;55(10):7758-7774.
9	Peptide aptamers that bind to geminivirus replication proteins confer a resistance phenotype to tomato yellow leaf curl virus and tomato mottle virus infection in tomato. J Virol. 2013 Sep;87(17):9691-706.
10	Targeting Inhibition of SmpB by Peptide Aptamer Attenuates the Virulence to Protect Zebrafish against Aeromonas veronii Infection. Front Microbiol. 2017 Sep 13;8:1766.
11	A hexapeptide motif that electrostatically binds to the surface of titanium. J Am Chem Soc. 2003 Nov 26;125(47):14234-5.
12	Inhibition of vaccinia virus replication by peptide aptamers. Antiviral Res. 2009 Jun;82(3):134-40.
13	Peptide aptamers targeting the hepatitis B virus core protein: a new class of molecules with antiviral activity. Oncogene. 2001 Oct 4;20(45):6579-86.
14	Peptide aptamers as new tools to modulate clathrin-mediated internalisation--inhibition of MT1-MMP internalisation. BMC Cell Biol. 2010 Jul 23;11:58.
15	NoPv1: a synthetic antimicrobial peptide aptamer targeting the causal agents of grapevine downy mildew and potato late blight. Sci Rep. 2020 Oct 16;10(1):17574.
16	Designing of peptide aptamer targeting the receptor-binding domain of spike protein of SARS-CoV-2: an in silico study. Mol Divers. 2021 Jan 2;1-13.
17	Isolation of peptide aptamers that inhibit intracellular processes. Proc Natl Acad Sci U S A. 2000 Feb 29;97(5):2241-6.
18	Selection of a novel peptide aptamer with high affinity for TiO 2-nanoparticle through a direct electroporation with TiO 2-binding phage complexes. J Biosci Bioeng. 2016 Nov;122(5):528-532.
19	Development of systemic in vitro evolution and its application to generation of peptide-aptamer-based inhibitors of cathepsin E. J Mol Biol. 2009 Apr 17;387(5):1186-98.
20	Selection of cyclic peptide aptamers to HCV IRES RNA using mRNA display. Proc Natl Acad Sci U S A. 2008 Oct 7;105(40):15293-8.
21	Induction of apoptosis in human papillomaviruspositive cancer cells by peptide aptamers targeting the viral E6 oncoprotein. Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6693-7.
22	PEP-on-DEP: A competitive peptide-based disposable electrochemical aptasensor for renin diagnostics. Biosens Bioelectron. 2016 Oct 15;84:120-5.
23	Targeting SOX2 Protein with Peptide Aptamers for Therapeutic Gains against Esophageal Squamous Cell Carcinoma. Mol Ther. 2020 Mar 4;28(3):901-913.
24	Reducing Allergenicity to Arginine Kinase from Mud Crab Using Site-Directed Mutagenesis and Peptide Aptamers. J Agric Food Chem. 2019 May 1;67(17):4958-4966.
25	A peptide aptamer to antagonize BCL-6 function. Oncogene. 2006 Apr 6;25(15):2223-33.
26	Targeting cyclin-dependent kinases in Drosophila with peptide aptamers. Proc Natl Acad Sci U S A. 1998 Nov 24;95(24):14266-71.
27	Peptide aptamers with binding specificity for the intracellular domain of the ErbB2 receptor interfere with AKT signaling and sensitize breast cancer cells to Taxol. Mol Cancer Res. 2006 Dec;4(12):983-98.
28	Peptides and aptamers targeting HSP70: a novel approach for anticancer chemotherapy. Cancer Res. 2011 Jan 15;71(2):484-95.
29	A new prostate carcinoma binding peptide (DUP-1) for tumor imaging and therapy. Clin Cancer Res. 2005 Jan 1;11(1):139-46.
30	Enhanced growth inhibition of prostate cancer in vitro and in vivo by a recombinant adenovirus-mediated dual-aptamer modified drug delivery system. Cancer Lett. 2016 Dec 28;383(2):230-242.
31	Inactivation of Ras function by allele-specific peptide aptamers. Oncogene. 2002 Aug 22;21(37):5753-7.
32	Selective inhibition of TGF-beta responsive genes by Smad-interacting peptide aptamers from FoxH1, Lef1 and CBP. Oncogene. 2005 Jun 2;24(24):3864-74.
33	Smad4 cooperates with lymphoid enhancer-binding factor 1/T cell-specific factor to increase c-myc expression in the absence of TGF-beta signaling. Proc Natl Acad Sci U S A. 2006 Dec 5;103(49):18580-5.
34	Increasing the range of drug targets: interacting peptides provide leads for the development of oncoprotein inhibitors. Bioengineered. Nov-Dec 2012;3(6):320-5.
35	The inhibition of stat5 by a Peptide aptamer ligand specific for the DNA binding domain prevents target gene transactivation and the growth of breast and prostate tumor cells. Pharmaceuticals (Basel). 2013 Aug 20;6(8):960-87.
36	Aptamer-derived peptides as potent inhibitors of the oncogenic RhoGEF Tgat. Chem Biol. 2009 Apr 24;16(4):391-400.
37	Peptide combinatorial libraries identify TSC2 as a death-associated protein kinase (DAPK) death domain-binding protein and reveal a stimulatory role for DAPK in mTORC1 signaling. J Biol Chem. 2009 Jan 2;284(1):334-344.