Details of the PSS

General Information of Protein Scaffold of SBP (PSS) (ID: PS011)
PSS Name	Anticalin
Synonyms	Engineered lipocalin; Lipocalin-based binder
Diagrammatic Sketch
Highest Status	Phase II
Class	Non-Antibody
Fold Type	One Alpha-Helix + Beta-Sheets + Loops
Thermal Denaturation TEMP	53-75℃
Sequence Length	120-199
Molecular Weight	13-28 kDa

Parental Protein of This PSS

Parental Protein Name	Synonym		PDB ID	UniProt ID	Origin
Lipocalin	Lcn		1BBP	P31025	Human; Pieris brassicae

Synthetic Binding Protein (SBP) Featuring this PSS

SBP Name	Highest Status	Template	Expression System	Selection Method	Details	Ref
Anticalin PRS-060	Phase II	N.A.	N.A.	N.A.	SBP Info	[1], []
Anticalin PRS-080	Phase II	N.A.	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[2], [1], [3]
Anticalin Angiocal	Phase I	N.A.	N.A.	N.A.	SBP Info	[2], [1], [4]
Anticalin Cinrebafusp alfa	Phase I	N.A.	N.A.	N.A.	SBP Info	[1], []
Anticalin anti-Abeta40 H1G1	Research	Lipocalin 2 (Lcn2)	Escherichia coli	Phage display	SBP Info	[5]
Anticalin anti-Abeta40 H1GA	Research	Lipocalin 2 (Lcn2)	Escherichia coli	Phage display	SBP Info	[5]
Anticalin anti-Abeta40 S1A4	Research	Lipocalin 2 (Lcn2)	Escherichia coli	Phage display	SBP Info	[5]
Anticalin anti-Abeta40 US7	Research	Lipocalin 2 (Lcn2)	Escherichia coli	Phage display	SBP Info	[5]
Anticalin anti-BzBuPht PhtA	Research	Bilin-binding protein	Escherichia coli JM83	Phage display	SBP Info	[6]
Anticalin anti-BzBuPht PhtB	Research	Bilin-binding protein	Escherichia coli JM83	Phage display	SBP Info	[6]
Anticalin anti-BzBuPht PhtC	Research	Bilin-binding protein	Escherichia coli JM83	Phage display	SBP Info	[6]
Anticalin anti-CD98 P3D11	Research	Lipocalin 2 (Lcn2)	Escherichia coli	Phage display	SBP Info	[7]
Anticalin anti-CTLA-4 PRS-010#001	Research	Lipocalin 2 (Lcn2)	Escherichia coli	Phage display	SBP Info	[8]
Anticalin anti-CTLA-4 PRS-010#002	Research	Lipocalin 2 (Lcn2)	Escherichia coli	Phage display	SBP Info	[8]
Anticalin anti-CTLA-4 PRS-010#003	Research	Lipocalin 2 (Lcn2)	Escherichia coli	Phage display	SBP Info	[8]
Anticalin anti-Digoxigenin DigA16	Research	Bilin-binding protein	Escherichia coli JM83	Phage display	SBP Info	[9], [10]
Anticalin anti-Digoxigenin DigA16/15	Research	Bilin-binding protein	Escherichia coli JM83	Phage display	SBP Info	[11]
Anticalin anti-Digoxigenin DigA16/16	Research	Bilin-binding protein	Escherichia coli JM83	Phage display	SBP Info	[11]
Anticalin anti-Digoxigenin DigA16/18	Research	Bilin-binding protein	Escherichia coli JM83	Phage display	SBP Info	[11]
Anticalin anti-Digoxigenin DigA16/19	Research	Bilin-binding protein	Escherichia coli JM83	Phage display	SBP Info	[11]
Anticalin anti-Digoxigenin DigA16/20	Research	Bilin-binding protein	Escherichia coli JM83	Phage display	SBP Info	[11]
Anticalin anti-Digoxigenin DigA16/22	Research	Bilin-binding protein	Escherichia coli JM83	Phage display	SBP Info	[11]
Anticalin anti-Digoxigenin DigA16/23	Research	Bilin-binding protein	Escherichia coli JM83	Phage display	SBP Info	[11]
Anticalin anti-Digoxigenin DigA16/24	Research	Bilin-binding protein	Escherichia coli JM83	Phage display	SBP Info	[11]
Anticalin anti-E2 clone A	Research	Bilin-binding protein	Escherichia coli BL21 (DE3)	Ribosome display	SBP Info	[12]
Anticalin anti-Fluorescein FluA	Research	Bilin-binding protein	Escherichia coli	Phage display	SBP Info	[1], [13]
Anticalin anti-HB HbgA	Research	Human apolipoprotein D (ApoD)	Escherichia coli	Phage display and Colony screening	SBP Info	[14]
Anticalin anti-Hsp70 BBG10	Research	Lipocalin 2 (Lcn2)	Escherichia coli	EspP-based Escherichia coli K12 display	SBP Info	[15]
Anticalin anti-Hsp70 BBG10C/I	Research	BBG10	Escherichia coli	EspP-based Escherichia coli K12 display	SBP Info	[15]
Anticalin anti-Hsp70 BBG10I	Research	BBG10	Escherichia coli	EspP-based Escherichia coli K12 display	SBP Info	[15]
Anticalin anti-Hsp70 BBG10I-1	Research	BBG10	Escherichia coli	EspP-based Escherichia coli K12 display	SBP Info	[15]
Anticalin anti-muTfR-ED T4B11	Research	human lipocalin 2Lcn2	E. coli	phage display	SBP Info	[16]
Anticalin anti-muTfR-ED T4B11LN6	Research	human lipocalin 2Lcn2	E. coli	bacterial cell surface display	SBP Info	[16]
Anticalin anti-PSMA A3	Research	Lipocalin 2 (Lcn2)	Escherichia coli	Phage display and ELISA screening	SBP Info	[17]
Anticalin anti-PSMA A3A5	Research	Lipocalin 2 (Lcn2)	Escherichia coli	Phage display and ELISA screening	SBP Info	[17]
Anticalin anti-PSMA A3A5.1	Research	Lipocalin 2 (Lcn2)	Escherichia coli	Phage display and ELISA screening	SBP Info	[17]
Anticalin anti-PSMA A3A5.7	Research	Lipocalin 2 (Lcn2)	Escherichia coli	Phage display and ELISA screening	SBP Info	[17]
Anticalin anti-VEGFR-3 A5C3	Research	Lipocalin 2 (Lcn2)	Escherichia coli	Phage display	SBP Info	[18]
Anticalin anti-VEGFR-3 A5C3(F71H)	Research	Lipocalin 2 (Lcn2)	Escherichia coli	Phage display	SBP Info	[18]
Anticalin anti-VEGFR-3 A5C3(F71Q/Y77H)	Research	Lipocalin 2 (Lcn2)	Escherichia coli	Phage display	SBP Info	[18]
Anticalin anti-VEGFR-3 S3A9	Research	Lipocalin 2 (Lcn2)	Escherichia coli	Phage display	SBP Info	[18]
Anticalin anti-VEGFR-3 U3D7	Research	Lipocalin 2 (Lcn2)	Escherichia coli	Phage display	SBP Info	[18]
Anticalin anti-CTLA-4 Lcn2m6	Research	N.A.	Escherichia coli	Escherichia coli display and Fluorescence-activated cell sorting	SBP Info	[19]
Anticalin anti-CTLA-4 Lcn2m7.1	Research	N.A.	Escherichia coli	Escherichia coli display and Fluorescence-activated cell sorting	SBP Info	[19]
Anticalin anti-Digoxigenin DigA	Research	N.A.	Escherichia coli JM83	Phage display	SBP Info	[11]
Anticalin anti-Fibronectin N10D	Research	Lipocalin 2 (Lcn2)	N.A.	Phage display	SBP Info	[1], [20], [21]
Anticalin anti-Fibronectin N7A	Research	Lipocalin 2 (Lcn2)	N.A.	Phage display	SBP Info	[1], [20], [21]
Anticalin anti-Fibronectin N7E	Research	Lipocalin 2 (Lcn2)	N.A.	Phage display	SBP Info	[1], [20], [21]
Anticalin anti-Fibronectin N9B	Research	Lipocalin 2 (Lcn2)	N.A.	Phage display	SBP Info	[1], [20], [21]
Anticalin anti-PQ clone 1	Research	N.A.	Escherichia coli BL21 (DE3)	Ribosome display	SBP Info	[22]
Anticalin anti-CTLA-4 PRS-010	Research	N.A.	N.A.	N.A.	SBP Info	[23]
Anticalin anti-HGFR PRS-110	Research	N.A.	N.A.	N.A.	SBP Info	[1], [24]

References

1	Recent advances in the development of novel protein scaffolds based therapeutics. Int J Biol Macromol. 2017 Sep;102:630-641.
2	In vitro-engineered non-antibody protein therapeutics. Protein Cell. 2018 Jan;9(1):3-14.
3	Sustained plasma hepcidin suppression and iron elevation by Anticalin-derived hepcidin antagonist in cynomolgus monkey. Br J Pharmacol. 2018 Apr;175(7):1054-1065.
4	Functional characterization of a VEGF-A-targeting Anticalin, prototype of a novel therapeutic human protein class. Angiogenesis. 2016 Jan;19(1):79-94.
5	High-affinity Anticalins with aggregation-blocking activity directed against the Alzheimer -amyloid peptide. Biochem J. 2016 Jun 1;473(11):1563-78.
6	Generation of anticalins with specificity for a nonsymmetric phthalic acid ester. Anal Biochem. 2002 Sep 15;308(2):269-77.
7	Development of a high affinity Anticalin ? directed against human CD98hc for theranostic applications. Theranostics. 2020 Jan 12;10(5):2172-2187.
8	An engineered lipocalin specific for CTLA-4 reveals a combining site with structural and conformational features similar to antibodies. Proc Natl Acad Sci U S A. 2009 May 19;106(20):8198-203.
9	Intravenous application of an anticalin dramatically lowers plasma digoxin levels and reduces its toxic effects in rats. Toxicol Appl Pharmacol. 2012 Sep 15;263(3):352-9.
10	Anticalins': a new class of engineered ligand-binding proteins with antibody-like properties. J Biotechnol. 2001 Jun;74(4):257-75.
11	Tuning ligand affinity, specificity, and folding stability of an engineered lipocalin variant -- a so-called 'anticalin' -- using a molecular random approach. Biophys Chem. 2002 May 2;96(2-3):213-28.
12	Construction of ribosome display library based on lipocalin scaffold and screening anticalins with specificity for estradiol. Analyst. 2012 May 21;137(10):2470-9.
13	Small antibody-like proteins with prescribed ligand specificities derived from the lipocalin fold. Proc Natl Acad Sci U S A. 1999 Mar 2;96(5):1898-903.
14	Construction of an artificial receptor protein ("anticalin") based on the human apolipoprotein D. Chembiochem. 2004 Feb 6;5(2):191-9.
15	Selection of an Anticalin? against the membrane form of Hsp70 via bacterial surface display and its theranostic application in tumour models. Biol Chem. 2018 Feb 23;399(3):235-252.
16	FerryCalin: an Engineered Lipocalin Protein Directed against the Transferrin Receptor with Potential for Brain Drug Delivery. Chembiochem. 2023 May 16;24(10):e202200795.
17	Selection and characterization of Anticalins targeting human prostate-specific membrane antigen (PSMA). Protein Eng Des Sel. 2016 Mar;29(3):105-15.
18	Anticalins directed against vascular endothelial growth factor receptor 3 (VEGFR-3) with picomolar affinities show potential for medical therapy and in vivo imaging. Biol Chem. 2017 Jan 1;398(1):39-55.
19	High-throughput sorting of an Anticalin library via EspP-mediated functional display on the Escherichia coli cell surface. J Mol Biol. 2010 Jul 23;400(4):783-802.
20	Anticalins directed against the fibronectin extra domain B as diagnostic tracers for glioblastomas. Int J Cancer. 2016 Mar 1;138(5):1269-80.
21	Combinatorial design of an Anticalin directed against the extra-domain b for the specific targeting of oncofetal fibronectin. J Mol Biol. 2013 Feb 22;425(4):780-802.
22	Selection of an artificial paraquat-specific binding protein from a ribosome display library based on a lipocalin scaffold. Biotechnol Appl Biochem. 2020 Nov 10.
23	Challenges and opportunities for non-antibody scaffold drugs. Drug Discov Today. 2015 Oct;20(10):1271-83.
24	In vivo visualization of MET tumor expression and anticalin biodistribution with the MET-specific anticalin 89Zr-PRS-110 PET tracer. J Nucl Med. 2014 Apr;55(4):665-71.