Details of the SBP

General Information of Synthetic Binding Protein (SBP) (ID: SBP000076)
SBP Name
Anticalin anti-Digoxigenin DigA16/19
Synonyms
Anticalin DigA16/19
Molecular Weight 13.7 kDa
Thermal Denaturation TEMP 62.5 ℃
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli JM83
Selection Method Phage display
Highest Status Research
Sequence Length 120
SBP Sequence
>Anticalin anti-Digoxigenin DigA16/19
WSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGKSVKVSRYSVIHGKEYFSEGTAYPVGD
SKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIGYFCRYDEDKKGHWDAVWVLSRSMVLT
3D Structure
Computationally Modelled Structure
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Template Name Bilin-binding protein
Protein Scaffold Information of This SBP
Scaffold ID PS011
Scaffold Info
[1]
Scaffold Name Anticalin
Scaffold Class Non-Antibody
Fold Type One Alpha-Helix + Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Digoxigenin
BTS Info
Binder Research tool Kd: 12.4 nM Technical University of Munich [1]
References
1 Tuning ligand affinity, specificity, and folding stability of an engineered lipocalin variant -- a so-called 'anticalin' -- using a molecular random approach. Biophys Chem. 2002 May 2;96(2-3):213-28.