Details of the BTS

General Information of Binding Target of SBP (BTS) (ID: ST00476)
BTS Name	Digoxigenin
Synonyms	DIG
BTS Type	Small Molecule

Synthetic Binding Protein (SBP) Targeting This BTS

SBP Name	Highest Status	Mechanism	Affinity	Application	Details	Ref
Anticalin anti-Digoxigenin DigA	Research	Binder	Kd: 295 nM	Research tool	SBP Info	[1]
Anticalin anti-Digoxigenin DigA16	Research	Binder	Kd: 30.2 nM	Digitalis intoxication	SBP Info	[2], [3]
Anticalin anti-Digoxigenin DigA16/15	Research	Binder	Kd: 27.2 nM	Research tool	SBP Info	[1]
Anticalin anti-Digoxigenin DigA16/16	Research	Binder	Kd: 22.5 nM	Research tool	SBP Info	[1]
Anticalin anti-Digoxigenin DigA16/18	Research	Binder	Kd: 13.9 nM	Research tool	SBP Info	[1]
Anticalin anti-Digoxigenin DigA16/19	Research	Binder	Kd: 12.4 nM	Research tool	SBP Info	[1]
Anticalin anti-Digoxigenin DigA16/20	Research	Binder	Kd: 48.2 nM	Research tool	SBP Info	[1]
Anticalin anti-Digoxigenin DigA16/22	Research	Binder	Kd: 47 nM	Research tool	SBP Info	[1]
Anticalin anti-Digoxigenin DigA16/23	Research	Binder	Kd: 29.2 nM	Research tool	SBP Info	[1]
Anticalin anti-Digoxigenin DigA16/24	Research	Binder	Kd: 20.3 nM	Research tool	SBP Info	[1]
scFv anti-DIG M-DIG1	Research	Inhibitor	N.A.	Research tool	SBP Info	[4]
scFv anti-DIG P-DIG3	Research	Inhibitor	N.A.	Research tool	SBP Info	[4]
scFv anti-Digoxigenin C8	Research	Binder	Kd: 7.2 nM	Research tool	SBP Info	[5]
scFv anti-Digoxigenin clone 1.7-1	Research	Binder	N.A.	Research tool	SBP Info	[6], [7]
scFv anti-Digoxigenin clone 22.5-1	Research	Binder	N.A.	Research tool	SBP Info	[6], [7]
scFv anti-Digoxigenin clone 22.5-2	Research	Binder	N.A.	Research tool	SBP Info	[6], [7]
scFv anti-Digoxigenin clone 22.5-3	Research	Binder	N.A.	Research tool	SBP Info	[6], [7]
scFv anti-Digoxigenin clone 26-10	Research	Binder	Kd: 2.3 nM	Tools for faster folding	SBP Info	[5]
scFv anti-Digoxigenin clone 3.8-1	Research	Binder	N.A.	Research tool	SBP Info	[6], [7]

References

1	Tuning ligand affinity, specificity, and folding stability of an engineered lipocalin variant -- a so-called 'anticalin' -- using a molecular random approach. Biophys Chem. 2002 May 2;96(2-3):213-28.
2	Intravenous application of an anticalin dramatically lowers plasma digoxin levels and reduces its toxic effects in rats. Toxicol Appl Pharmacol. 2012 Sep 15;263(3):352-9.
3	Anticalins': a new class of engineered ligand-binding proteins with antibody-like properties. J Biotechnol. 2001 Jun;74(4):257-75.
4	Two ScFv antibody libraries derived from identical VL-VH framework with different binding site designs display distinct binding profiles. Protein Eng Des Sel. 2013 Oct;26(10):683-93.
5	A scFv antibody mutant isolated in a genetic screen for improved export via the twin arginine transporter pathway exhibits faster folding. J Mol Biol. 2007 Jun 8;369(3):631-9.
6	Quantitative analysis of the effect of the mutation frequency on the affinity maturation of single chain Fv antibodies. Proc Natl Acad Sci U S A. 2000 Feb 29;97(5):2029-34.
7	In vitro scanning saturation mutagenesis of all the specificity determining residues in an antibody binding site. Protein Eng. 1999 Apr;12(4):349-56.