General Information of Synthetic Binding Protein (SBP) (ID: SBP001647)
SBP Name
scFv anti-Digoxigenin clone 22.5-1
Synonyms
scFv 22.5-1
Molecular Weight 26.2 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli BL21 (DE3)
Selection Method Escherichia coli display
Highest Status Research
Sequence Length 248
SBP Sequence
>scFv anti-Digoxigenin clone 22.5-1
HMEVQLQQSGPELVKPGASVRMSCKSSGYTSTDFYMNWVRQSHGKSLDYIGYISPYSGVT
GYNQKFKGKATLTVDKSSSTAYMELRSLTSEDSAVYYCAGSSGNKNKAMDYWGHGASVTV
SSGGGGSGGGGSGGGGSDIVLTQSPASLAVSLGQRATISCRSSQSLVHSNGNTYRNWYQQ
KPGQPPKLLIYKVSNRFSGVPARFSGSGSVSDFTLTIDPVGEDDAAIYYCSQTTHVPPTF
GSGTKLEL
3D Structure
Computationally Modelled Structure
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Template Name scFv 26-10
Protein Scaffold Information of This SBP
Scaffold ID PS057
Scaffold Info
[1] , [2]
Scaffold Name scFv
Scaffold Class Antibody fragment
Fold Type Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Digoxigenin
BTS Info
Binder Research tool N.A. University of Texas at Austin [1] , [2]
References
1 Quantitative analysis of the effect of the mutation frequency on the affinity maturation of single chain Fv antibodies. Proc Natl Acad Sci U S A. 2000 Feb 29;97(5):2029-34.
2 In vitro scanning saturation mutagenesis of all the specificity determining residues in an antibody binding site. Protein Eng. 1999 Apr;12(4):349-56.