Details of the SBP | SYNBIP

General Information of Synthetic Binding Protein (SBP) (ID: SBP001648)
SBP Name	scFv anti-Digoxigenin clone 22.5-2
Synonyms	scFv 22.5-2
Molecular Weight	26.4 kDa
Design Method	Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System	Escherichia coli BL21 (DE3)
Selection Method	Escherichia coli display
Highest Status	Research
Sequence Length	248
SBP Sequence	>scFv anti-Digoxigenin clone 22.5-2 HMEVQLEQSGPELVKPGASVRMSCKFSGYISTDFYMNWVRQSHGKSLDYIGYISPYSGIT GYNQKFKGKATLTVDKSSSTAYMELRSLTSEDSAVYYCAGSSGNKNKAMDYWGHGASVTV SSGGGGSSGGGSGGGGSDIVLTQSPASLAVSLGQRVTISCRFSQSLVHSNGNTYLNWYQQ KPGQPPKLLIYKVSNRFSGVPARFSGSGSESDFTLTIDPVGEDDAAIYYCSQTTHVPPTF GSGTKLEL
3D Structure
	Computationally Modelled Structure
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Template Name	scFv 26-10

Protein Scaffold Information of This SBP
Scaffold ID	PS057 Scaffold Info					[1] , [2]
Scaffold Name	scFv
Scaffold Class	Antibody fragment
Fold Type	Beta-Sheets + Loops

Binding Target(s) of This SBP (BTS)

BTS Name	Details	Mechanism	Application	Affinity	Research Organization	Ref
Digoxigenin	BTS Info	Binder	Research tool	N.A.	University of Texas at Austin	[1] , [2]

References

1	Quantitative analysis of the effect of the mutation frequency on the affinity maturation of single chain Fv antibodies. Proc Natl Acad Sci U S A. 2000 Feb 29;97(5):2029-34.
2	In vitro scanning saturation mutagenesis of all the specificity determining residues in an antibody binding site. Protein Eng. 1999 Apr;12(4):349-56.