Details of the SBP
| General Information of Synthetic Binding Protein (SBP) (ID: SBP000068) | ||||||
|---|---|---|---|---|---|---|
| SBP Name |
Anticalin anti-Digoxigenin DigA16
|
|||||
| Synonyms |
Anticalin DigA16
|
|||||
| Molecular Weight | 13.7 kDa | |||||
| Thermal Denaturation TEMP | 70.6 ℃ | |||||
| Design Method | Traditional methods (Site-directed mutagenesis and/or Directed evolution) | |||||
| Expression System | Escherichia coli JM83 | |||||
| Selection Method | Phage display | |||||
| Highest Status | Research | |||||
| PDB ID | 1LNM; 1LKE; 1KXO | |||||
| Sequence Length | 120 | |||||
| SBP Sequence |
>Anticalin anti-Digoxigenin DigA16
WSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGKSVKVSRYSVIHGKEYFSEGTAYPVGD SKIGKIYHSYTIGGVTQEGVFNVLSTDNKNYIIGYFCSYDEDKKGHMDLVWVLSRSMVLT |
|||||
| 3D Structure | ||||||
| Experimentally Validated Structure | ||||||
| Click to Save PDB File | ||||||
| Template Name | Bilin-binding protein | |||||
| Protein Scaffold Information of This SBP | ||||||
|---|---|---|---|---|---|---|
| Scaffold ID | PS011 | [1] , [2] | ||||
| Scaffold Name | Anticalin | |||||
| Scaffold Class | Non-Antibody | |||||
| Fold Type | One Alpha-Helix + Beta-Sheets + Loops | |||||
| Binding Target(s) of This SBP (BTS) |
|---|
| BTS Name | Details | Mechanism | Application | Affinity | Research Organization | Ref |
|---|---|---|---|---|---|---|
| Digoxigenin | Binder | Digitalis intoxication | Kd: 30.2 nM | Technical University of Munich | [1] , [2] | |
| References |
|---|