General Information of Synthetic Binding Protein (SBP) (ID: SBP001790)
SBP Name
scFv anti-DIG P-DIG3
Synonyms
scFv P-DIG3
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli MC1061 F
Selection Method Phage display
Highest Status Research
SBP Sequence
>VH CDR1
SYYMH
>VH CDR2
YISPVTGETD
>VH CDR3
ARGLPSGYYWYAYFDY
>VL CDR1
ALA
>VL CDR3
QQSYSTPW
Template Name Human germline VH3 (IGHV3-23/DP-47) and Vgamma3 (IGKV3-20/A27)
Protein Scaffold Information of This SBP
Scaffold ID PS057
Scaffold Info
[1]
Scaffold Name scFv
Scaffold Class Antibody fragment
Fold Type Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Digoxigenin
BTS Info
Inhibitor Research tool N.A. University of Turku [1]
References
1 Two ScFv antibody libraries derived from identical VL-VH framework with different binding site designs display distinct binding profiles. Protein Eng Des Sel. 2013 Oct;26(10):683-93.