General Information of Synthetic Binding Protein (SBP) (ID: SBP001657)
SBP Name
scFv anti-Digoxigenin clone 26-10
Synonyms
scFv 26-10
Molecular Weight 26.3 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli FA113
Highest Status Research
Sequence Length 248
SBP Sequence
>scFv anti-Digoxigenin clone 26-10
HMEVQLQQSGPELVKPGASVRMSCKSSGYIFTDFYMNWVRQSHGKSLDYIGYISPYSGVT
GYNQKFKGKATLTVDKSSSTAYMELRSLTSEDSAVYYCAGSSGNKNKAMDYWGHGASVTV
SSGGGGSGGGGSGGGGSDIVLTQSPASLAVSLGQRATISCRSSQSLVHSNGNTYLNWYQQ
KPGQPPKLLIYKVSNRFSGVPARFSGSGSESDFTLTIDPVEEDDAAIYYCSQTTHVPPTF
GSGTKLEL
3D Structure
Computationally Modelled Structure
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Protein Scaffold Information of This SBP
Scaffold ID PS057
Scaffold Info
[1]
Scaffold Name scFv
Scaffold Class Antibody fragment
Fold Type Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Digoxigenin
BTS Info
Binder Tools for faster folding Kd: 2.3 nM University of Texas [1]
References
1 A scFv antibody mutant isolated in a genetic screen for improved export via the twin arginine transporter pathway exhibits faster folding. J Mol Biol. 2007 Jun 8;369(3):631-9.