General Information of Synthetic Binding Protein (SBP) (ID: SBP000101)
SBP Name
Anticalin anti-CTLA-4 PRS-010#003
Synonyms
Anticalin PRS-010#003
Molecular Weight 20.2 kDa
Thermal Denaturation TEMP 65.5 ℃
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli
Selection Method Phage display
Highest Status Research
Sequence Length 178
SBP Sequence
>Anticalin anti-CTLA-4 PRS-010#003
QDSTSDLIPAPPLSKVPLQQNFQDNQFHGKWYVVGLAGNRILRDDQHPMNMYATIYELKE
DKSYNVTSVISSHKKCEYTIATFVPGSQPGEFTLGNIKSYGDKTSYLVRVVSTDYNQYAM
VFFKLAEDNAEFFAITIYGRTKELASELKENFIRFSKSLGLPENHIVFPVPIDQCIDG
3D Structure
Computationally Modelled Structure
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Template Name Lipocalin 2 (Lcn2)
Protein Scaffold Information of This SBP
Scaffold ID PS011
Scaffold Info
[1]
Scaffold Name Anticalin
Scaffold Class Non-Antibody
Fold Type One Alpha-Helix + Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Cytotoxic T-lymphocyte protein 4
BTS Info
Binder Immunotherapy of cancer and infectious disease Kd: 9 nM Technical University of Munich [1]
References
1 An engineered lipocalin specific for CTLA-4 reveals a combining site with structural and conformational features similar to antibodies. Proc Natl Acad Sci U S A. 2009 May 19;106(20):8198-203.