General Information of Synthetic Binding Protein (SBP) (ID: SBP000080)
SBP Name
Anticalin anti-Hsp70 BBG10I
Synonyms
Anticalin BBG10I
Molecular Weight 20.7 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli
Selection Method EspP-based Escherichia coli K12 display
Highest Status Research
Sequence Length 178
SBP Sequence
>Anticalin anti-Hsp70 BBG10I
QDSTSDLIPAPPLSKVPLQQNFQDNQFhGKWYVVGLAGNHFLWEDKDPPKMYATIYELKE
DKSYNVTYVRFELKKCEYFIHTFVPGsQPGEFTLGQIKSRPDMTSVLVRVVSTNYNQHAM
VFFKRVIQNREYFQITLYGRTKELTSELKENFIRFSKSLGLPENHIVFPVPIDQCIDG
Template Name BBG10
Protein Scaffold Information of This SBP
Scaffold ID PS011
Scaffold Info
[1]
Scaffold Name Anticalin
Scaffold Class Non-Antibody
Fold Type One Alpha-Helix + Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Heat shock 70 kDa protein 1A
BTS Info
Binder Tumors [ICD-11: XH1N44] Kd: 2.2 nM Technical University of Munich [1]
References
1 Selection of an Anticalin? against the membrane form of Hsp70 via bacterial surface display and its theranostic application in tumour models. Biol Chem. 2018 Feb 23;399(3):235-252.