General Information of Synthetic Binding Protein (SBP) (ID: SBP000083)
SBP Name
Anticalin anti-PSMA A3A5
Synonyms
Anticalin A3A5
Molecular Weight 20.3 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli
Selection Method Phage display and ELISA screening
Highest Status Research
Sequence Length 178
SBP Sequence
>Anticalin anti-PSMA A3A5
QDSTSDLIPAPPLSKVPLQQNFQDNQFhGKWYVVGLAGNVILREDKDPYKMSATIYELKE
DKSYNVTNVRFYLKKCYYTIATFVPGsQPGEFTLGTIKSGPGKTSGLVRVVSTNYNQHAM
VFFKEVQQNREWFIITLYGRTKELTSELKENFIRFSKSLGLPENHIVFPVPIDQCIDG
Template Name Lipocalin 2 (Lcn2)
Protein Scaffold Information of This SBP
Scaffold ID PS011
Scaffold Info
[1]
Scaffold Name Anticalin
Scaffold Class Non-Antibody
Fold Type One Alpha-Helix + Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Glutamate carboxypeptidase 2
BTS Info
Binder Solid tumour/cancer [ICD-11: 2A00-2F9Z] Kd: 2.5 nM Academy of Sciences of the Czech Republic [1]
References
1 Selection and characterization of Anticalins targeting human prostate-specific membrane antigen (PSMA). Protein Eng Des Sel. 2016 Mar;29(3):105-15.