Details of the SBP

General Information of Synthetic Binding Protein (SBP) (ID: SBP000066)
SBP Name
Anticalin anti-Fluorescein FluA
Synonyms
Anticalin FluA
Molecular Weight 21.0 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli
Selection Method Phage display
Highest Status Research
PDB ID 1N0S
Sequence Length 184
SBP Sequence
>Anticalin anti-Fluorescein FluA
DVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGKSVKVSRYD
VIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIGYSCRYDED
KKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKVNNSNWSHP
QFEK
3D Structure
Experimentally Validated Structure
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Template Name Bilin-binding protein
Protein Scaffold Information of This SBP
Scaffold ID PS011
Scaffold Info
[1] , [2]
Scaffold Name Anticalin
Scaffold Class Non-Antibody
Fold Type One Alpha-Helix + Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Fluorescein
BTS Info
Binder Tools for payload delivery Kd: 35.2 nM Technical University of Munich [1] , [2]
References
1 Recent advances in the development of novel protein scaffolds based therapeutics. Int J Biol Macromol. 2017 Sep;102:630-641.
2 Small antibody-like proteins with prescribed ligand specificities derived from the lipocalin fold. Proc Natl Acad Sci U S A. 1999 Mar 2;96(5):1898-903.