General Information of Synthetic Binding Protein (SBP) (ID: SBP000092)
SBP Name
Anticalin anti-E2 clone A
Synonyms
Anticalin E2-A
Molecular Weight 20 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli BL21 (DE3)
Selection Method Ribosome display
Highest Status Research
SBP Sequence
>Anticalin anti-E2 clone A
NVYHDGACPEVKPVDNFDWSNYHGKWWEVAKYPFLLSKYGKCGWAEYTPEGKSVKVSKYF
VIHGKEYFNEGTAYPVGDSKIGKIYHKRTTGGTTMEFVFNVLSTDNKNYIIGYYCKYDED
KKGHRDTVWVLSRSKVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKVN
3D Structure
Computationally Modelled Structure
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Template Name Bilin-binding protein
Protein Scaffold Information of This SBP
Scaffold ID PS011
Scaffold Info
[1]
Scaffold Name Anticalin
Scaffold Class Non-Antibody
Fold Type One Alpha-Helix + Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Estradiol-17beta
BTS Info
Inhibitor Tools for detecting E(2) Kd: 54 nM Tian Jin University [1]
References
1 Construction of ribosome display library based on lipocalin scaffold and screening anticalins with specificity for estradiol. Analyst. 2012 May 21;137(10):2470-9.