General Information of Binding Target of SBP (BTS) (ID: ST00374)
BTS Name
Estradiol-17beta
Synonyms
E2
BTS Type
Small Molecule
Synthetic Binding Protein (SBP) Targeting This BTS
SBP Name Highest Status Mechanism Affinity Application Details Ref
Anticalin anti-E2 clone A Research Inhibitor Kd: 54 nM Tools for detecting E(2)
SBP Info
[1]
scFv anti-E2 m1-e7 Research Binder Kd: 3.8 nM Diagnostic reagent
SBP Info
[2], [3], [4]
scFv anti-E2 m2-c4 Research Binder Kd: 1.6 nM Diagnostic reagent
SBP Info
[2], [3]
scFv anti-E2 m3-a18 Research Binder Kd: 0.08 nM Diagnostic reagents
SBP Info
[5], [2]
scFv anti-E2 m3-b12 Research Binder Kd: 0.2 nM Diagnostic reagent
SBP Info
[2]
References
1 Construction of ribosome display library based on lipocalin scaffold and screening anticalins with specificity for estradiol. Analyst. 2012 May 21;137(10):2470-9.
2 "Breeding" diagnostic antibodies for higher assay performance: a 250-fold affinity-matured antibody mutant targeting a small biomarker. Anal Chem. 2013 May 21;85(10):4930-7.
3 Two-step in vitro antibody affinity maturation enables estradiol-17beta assays with more than 10-fold higher sensitivity. Anal Chem. 2010 Feb 1;82(3):1027-38.
4 Anti-estradiol-17beta single-chain Fv fragments: Generation, characterization, gene randomization, and optimized phage display. Steroids. 2008 Dec 22;73(14):1485-99.
5 Seeking high-priority mutations enabling successful antibody-breeding: systematic analysis of a mutant that gained over 100-fold enhanced affinity. Sci Rep. 2020 Mar 16;10(1):4807.