General Information of Synthetic Binding Protein (SBP) (ID: SBP001614)
SBP Name
scFv anti-E2 m3-a18
Synonyms
scFv m3-a18; scFv M3rd
Molecular Weight 26.1 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli XL1-Blue
Selection Method Phage display
Highest Status Research
Sequence Length 246
SBP Sequence
>scFv anti-E2 m3-a18
EVPLVESGGGLVKPGGSLRLSCTASGFPFSRSAMSWVRQSPDKRLEWVAEISSGGFFTSY
VDTVTGRFTISRDNAKNILYLEMSSLRPGDTAIYYCARERGIHYYGSSEIEDYWGQGTSV
TVSSGGGGSGGGGSGGGGSDILMTQTPSSMSVSLGDTVTITCHASRGVRSYIGWMQQKPG
KSFKGLIYNGTNLEDGISSRFGGRGSGTDYSLTISGLESEDFGDYYCVQYAQFPYTFGGG
TKLEVK
3D Structure
Computationally Modelled Structure
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Template Name scFv E4-4
Protein Scaffold Information of This SBP
Scaffold ID PS057
Scaffold Info
[1] , [2]
Scaffold Name scFv
Scaffold Class Antibody fragment
Fold Type Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Estradiol-17beta
BTS Info
Binder Diagnostic reagents Kd: 0.08 nM Kobe Pharmaceutical University [1] , [2]
References
1 Seeking high-priority mutations enabling successful antibody-breeding: systematic analysis of a mutant that gained over 100-fold enhanced affinity. Sci Rep. 2020 Mar 16;10(1):4807.
2 "Breeding" diagnostic antibodies for higher assay performance: a 250-fold affinity-matured antibody mutant targeting a small biomarker. Anal Chem. 2013 May 21;85(10):4930-7.