General Information of Synthetic Binding Protein (SBP) (ID: SBP000103)
SBP Name
Anticalin anti-BzBuPht PhtB
Synonyms
Anticalin PhtB
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli JM83
Selection Method Phage display
Highest Status Research
SBP Sequence
>Anticalin anti-BzBuPht PhtB
NVYHDGACPEVKPVDNFDWSNYHGKWWEVAKYPTQGDKYGKCGWAEYTPEGKSVKVSSYR
VIHGKEYFTEGTAYPVGDSKIGKIYHKVTYGGGTHELVFNVLSTDNKNYIIGYVCFYDED
KKGHLDAVWVLSRSKVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKVN
3D Structure
Computationally Modelled Structure
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Template Name Bilin-binding protein
Protein Scaffold Information of This SBP
Scaffold ID PS011
Scaffold Info
[1]
Scaffold Name Anticalin
Scaffold Class Non-Antibody
Fold Type One Alpha-Helix + Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Benzyl butyl phthalate
BTS Info
Binder Tools as a reagent specificity for a nonsymmetric phthalic acid ester Kd: 6200 nM Technical University of Munich [1]
References
1 Generation of anticalins with specificity for a nonsymmetric phthalic acid ester. Anal Biochem. 2002 Sep 15;308(2):269-77.