Details of the BTS

General Information of Binding Target of SBP (BTS) (ID: ST00368)
BTS Name	Hen egg-white lysozyme
Synonyms	HEWL
BTS Type	Protein
Organism	Gallus gallus (Chicken)

Synthetic Binding Protein (SBP) Targeting This BTS

SBP Name	Highest Status	Mechanism	Affinity	Application	Details	Ref
Affitin anti-DNA/HEWL mL3	Research	Binder	Kd: 6700 nM	Research tool	SBP Info	[1]
Affitin anti-HEWL H4	Research	Inhibitor	Kd: 11 nM	Tools for inhibiting of glycosidases	SBP Info	[2]
Affitin anti-HEWL H4S	Research	Binder	N.A.	Tools as a reagentless fluorescent biosensor	SBP Info	[3]
Affitin anti-HEWL M1	Research	Binder	Kd: 4.0 nM	Research tool	SBP Info	[4]
Affitin anti-HEWL M2	Research	Binder	Kd: 440 nM	Research tool	SBP Info	[4]
Affitin anti-HEWL M3	Research	Binder	Kd: 7.5 nM	Research tool	SBP Info	[4]
beta Roll domain anti-HEWL P101	Research	Binder	Kd: 3200 nM	Tools for controlled biomolecular recognition	SBP Info	[5]
beta Roll domain anti-HEWL PN206	Research	Binder	Kd: 6200 nM	Tools for controlled biomolecular recognition	SBP Info	[5]
beta Roll domain anti-HEWL PN406	Research	Binder	Kd: 2300 nM	Tools for controlled biomolecular recognition	SBP Info	[5]
beta Roll domain anti-HEWL PN406/PN406	Research	Binder	Kd: 3100 nM	Tools for controlled biomolecular recognition	SBP Info	[5]
beta Roll domain anti-HEWL PN715	Research	Binder	Kd: 7200 nM	Tools for controlled biomolecular recognition	SBP Info	[5]
beta Roll domain anti-HEWL WT/PN406	Research	Binder	Kd: 34000 nM	Tools for controlled biomolecular recognition	SBP Info	[5]
Diabody anti-HEWL (VHD1.3-VLD1.3)2	Research	Binder	N.A.	Tools for constructing small bivalent and bispecific antibody fragments in bacteria	SBP Info	[6]
Evibody anti-HEWL clys12	Research	Binder	N.A.	Cancers [ICD-11: 2D4Z]	SBP Info	[7]
Fab anti-HEWL HyHEL10-R5	Research	binder	Kd: 0.0028 nM	Research tool	SBP Info	[8]
Gp2-based binder anti-HEWL GalphaLysA(0.3.3)	Research	Binder	Kd: 0.9 nM	Research tool	SBP Info	[9]
Megabody anti-HEWL Mb-cAbLys3-cHopQ	Research	Binder	Kd: 2.9 nM	Research tool	SBP Info	[10]
Megabody anti-HEWL Mb-cAbLys3-cYgKE2	Research	Binder	Kd: 2.9 nM	Research tool	SBP Info	[10]
Neocarzinostatin-based binder anti-HEWL NCS-CDR3	Research	Binder	Kd: 500 nM	Cancers [ICD-11: 2D4Z]	SBP Info	[11]
OBody anti-HEWL AM1L10	Research	Inhibitor	Kd: 5000 nM	Tools for molecular recognition	SBP Info	[12]
OBody anti-HEWL AM2EP06	Research	Inhibitor	Kd: 250 nM	Tools for molecular recognition	SBP Info	[12]
OBody anti-HEWL AM3L09	Research	Inhibitor	Kd: 3 nM	Tools for molecular recognition	SBP Info	[12]
OBody anti-HEWL AM3L15	Research	Inhibitor	Kd: 30 nM	Tools for molecular recognition	SBP Info	[12]
OBody anti-HEWL NL8	Research	Inhibitor	Kd: 35000 nM	Tools for molecular recognition	SBP Info	[12]
Repebody anti-HEWL	Research	Binder	Kd: 3700 nM	Research tool	SBP Info	[13]
VNAR anti-HEWL 4F11	Research	Binder	Kd: 20 nM	Research tool	SBP Info	[14]
VNAR anti-HEWL 5A7	Research	Binder	Kd: 22 nM	Research tool	SBP Info	[14]
VNAR anti-HEWL A30V	Research	Binder	Kd: 10 nM	Research tool	SBP Info	[15]
VNAR anti-HEWL clone 754-1	Research	Binder	N.A.	Research tool	SBP Info	[16]
VNAR anti-HEWL clone 754-2	Research	Binder	N.A.	Research tool	SBP Info	[16]
VNAR anti-HEWL clone 853-4	Research	Binder	N.A.	Research tool	SBP Info	[16]
VNAR anti-HEWL clone 951-1	Research	Binder	N.A.	Research tool	SBP Info	[16]
VNAR anti-HEWL Epi6	Research	Binder	Kd: 8.5 nM	Research tool	SBP Info	[15]
VNAR anti-HEWL Ly-X20	Research	Binder	N.A.	Research tool	SBP Info	[17]
VNAR anti-HEWL PBLA8	Research	Binder	Kd: 1 nM	Research tool	SBP Info	[15]
VNAR anti-HEWL PBLB11	Research	Binder	Kd: 5 nM	Research tool	SBP Info	[15]
VNAR anti-HEWL PBLB5	Research	Binder	Kd: 30 nM	Research tool	SBP Info	[15]
VNAR anti-HEWL S61R	Research	Binder	Kd: 3.3 nM	Research tool	SBP Info	[15]
VNAR anti-HEWL tri-mutant	Research	Binder	Kd: 0.46 nM	Research tool	SBP Info	[18]

References

1	Affinity transfer to the archaeal extremophilic Sac7d protein by insertion of a CDR. Protein Eng Des Sel. 2014 Oct;27(10):431-8.
2	Potent and specific inhibition of glycosidases by small artificial binding proteins (affitins). PLoS One. 2014 May 13;9(5):e97438.
3	Reagentless fluorescent biosensors from artificial families of antigen binding proteins. Biosens Bioelectron. 2011 Jun 15;26(10):4184-90.
4	Characterization of Affitin proteolytic digestion in biorelevant media and improvement of their stabilities via protein engineering. Sci Rep. 2020 Nov 12;10(1):19703.
5	Catch and Release: Engineered Allosterically Regulated -Roll Peptides Enable On/Off Biomolecular Recognition. ACS Synth Biol. 2017 Sep 15;6(9):1732-1741.
6	"Diabodies": small bivalent and bispecific antibody fragments. Proc Natl Acad Sci U S A. 1993 Jul 15;90(14):6444-8.
7	Ribosome display and affinity maturation: from antibodies to single V-domains and steps towards cancer therapeutics. J Immunol Methods. 2001 Feb 1;248(1-2):31-45.
8	Arginine cluster introduction on framework region in anti-lysozyme antibody improved association rate constant by changing conformational diversity of CDR loops. Protein Sci. 2023 Sep;32(9):e4745.
9	A 45-Amino-Acid Scaffold Mined from the PDB for High-Affinity Ligand Engineering. Chem Biol. 2015 Jul 23;22(7):946-56.
10	Megabodies expand the nanobody toolkit for protein structure determination by single-particle cryo-EM. Nat Methods. 2021 Jan;18(1):60-68.
11	Affinity transfer by CDR grafting on a nonimmunoglobulin scaffold. Protein Sci. 2004 Jul;13(7):1882-91.
12	Tracking molecular recognition at the atomic level with a new protein scaffold based on the OB-fold. PLoS One. 2014 Jan 20;9(1):e86050.
13	Design of a binding scaffold based on variable lymphocyte receptors of jawless vertebrates by module engineering. Proc Natl Acad Sci U S A. 2012 Feb 28;109(9):3299-304.
14	Selection and characterization of naturally occurring single-domain (IgNAR) antibody fragments from immunized sharks by phage display. Mol Immunol. 2003 Sep;40(1):25-33.
15	First molecular and biochemical analysis of in vivo affinity maturation in an ectothermic vertebrate. Proc Natl Acad Sci U S A. 2006 Feb 7;103(6):1846-51.
16	Construction of an artificially randomized IgNAR phage display library: screening of variable regions that bind to hen egg white lysozyme. Mar Biotechnol (NY). 2013 Feb;15(1):56-62.
17	Rapid isolation of IgNAR variable single-domain antibody fragments from a shark synthetic library. Mol Immunol. 2007 Jan;44(4):656-65.
18	Dissection of the IgNAR V domain: molecular scanning and orthologue database mining define novel IgNAR hallmarks and affinity maturation mechanisms. J Mol Biol. 2010 Jul 9;400(2):155-70.