General Information of Synthetic Binding Protein (SBP) (ID: SBP000648)
SBP Name
Affitin anti-DNA/HEWL mL3
Synonyms
Affitin mL3
Molecular Weight 8.8 kDa
Thermal Denaturation TEMP 60.9 ℃
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli DH5 (alpha)
Highest Status Research
Sequence Length 76
SBP Sequence
>Affitin anti-DNA/HEWL mL3
MVKVKFKYKGEEKEVDTSKIKKVWRVGKMVSFTYDDNSTIYASYYESGKTGRGAVSEKDA
PKELLDMLARAEREKK
3D Structure
Computationally Modelled Structure
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Template Name Sac7d
Protein Scaffold Information of This SBP
Scaffold ID PS007
Scaffold Info
[1]
Scaffold Name Affitin
Scaffold Class Non-Antibody
Fold Type One Alpha-Helix + Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Hen egg-white lysozyme
BTS Info
Binder Research tool Kd: 6700 nM Nantes University [1]
DNA
BTS Info
Binder Research tool Kd: 62500 nM Nantes University [1]
References
1 Affinity transfer to the archaeal extremophilic Sac7d protein by insertion of a CDR. Protein Eng Des Sel. 2014 Oct;27(10):431-8.