Details of the PSS

General Information of Protein Scaffold of SBP (PSS) (ID: PS007)
PSS Name	Affitin
Synonyms	Nanofitins
Diagrammatic Sketch
Highest Status	Research
Class	Non-Antibody
Fold Type	One Alpha-Helix + Beta-Sheets + Loops
Thermal Denaturation TEMP	60-100℃
Sequence Length	60-76
Molecular Weight	6-9 kDa

Parental Protein of This PSS

Parental Protein Name	Synonym	PDB ID	UniProt ID	Origin
DNA-binding protein Aho7c	DNA-binding protein 7c; 7 kDa DNA-binding protein c; Aho7c	N.A.	F4B8X5	Acidianus hospitalis
DNA-binding protein Sac7d	Sac7d	1AZP	P13123	Sulfolobus acidocaldarius
DNA-binding protein Sso7d	Sso7d	1BBX	P39476	Sulfolobus solfataricus

Synthetic Binding Protein (SBP) Featuring this PSS

SBP Name	Highest Status	Template	Expression System	Selection Method	Details	Ref
Affitin anti-CelD E12	Research	Sac7d	Escherichia coli	Ribosome display	SBP Info	[1]
Affitin anti-CelD H3	Research	Sac7d	Escherichia coli	Ribosome display	SBP Info	[1]
Affitin anti-EpCAM A2	Research	Aho7c	Escherichia coli	Ribosome display	SBP Info	[2]
Affitin anti-EpCAM B10	Research	Aho7c WT	Escherichia coli DH5 (alpha) Iq	Ribosome display	SBP Info	[2]
Affitin anti-EpCAM C7	Research	Aho7c	Escherichia coli	Ribosome display	SBP Info	[2]
Affitin anti-HEWL H4	Research	Sac7d	Escherichia coli	Ribosome display	SBP Info	[1]
Affitin anti-IgG C3	Research	Sac7d	Escherichia coli	Ribosome display	SBP Info	[3]
Affitin anti-IgG D1	Research	Sac7d	Escherichia coli	Ribosome display	SBP Info	[3]
Affitin anti-IgG L28K	Research	Affitin D1	Escherichia coli	Ribosome display	SBP Info	[3]
Affitin anti-IgG N44A	Research	Affitin C3	Escherichia coli	Ribosome display	SBP Info	[3]
Affitin anti-SasA C5	Research	Sac7d	Escherichia coli	Ribosome display	SBP Info	[4]
Affitin anti-BSA Sso7d\2A8	Research	Sso7d	N.A.	Phage display	SBP Info	[5]
Affitin anti-DNA/HEWL mL3	Research	Sac7d	Escherichia coli DH5 (alpha)	N.A.	SBP Info	[6]
Affitin anti-GFP D8	Research	N.A.	Escherichia coli	Ribosome display	SBP Info	[7]
Affitin anti-GFP Sso7d\6B9	Research	Sso7d	N.A.	Phage display	SBP Info	[5]
Affitin anti-HEWL M1	Research	Affitin H4	Escherichia coli	N.A.	SBP Info	[8]
Affitin anti-HEWL M2	Research	Affitin H4	Escherichia coli	N.A.	SBP Info	[8]
Affitin anti-HEWL M3	Research	Affitin H4	Escherichia coli	N.A.	SBP Info	[8]
Affitin anti-IgG D1Sac7d	Research	Sac7d	Escherichia coli	N.A.	SBP Info	[9]
Affitin anti-IgG D1Sso7d	Research	Sac7d	Escherichia coli	N.A.	SBP Info	[9]
Affitin anti-IgG D1Sso7d (E36L)	Research	Sac7d	Escherichia coli	N.A.	SBP Info	[9]
Affitin anti-IgG D1Sso7d (K13L)	Research	Sac7d	Escherichia coli	N.A.	SBP Info	[9]
Affitin anti-IgG D1Sso7d DM	Research	Sac7d	Escherichia coli	N.A.	SBP Info	[9]
Affitin anti-Neutravidin Sso7d\2D6	Research	Sso7d	N.A.	Phage display	SBP Info	[5]
Affitin anti-PulD Sac7*33	Research	Sac7d	N.A.	Ribosome display	SBP Info	[10], [11]
Affitin anti-PulD Sac7*39	Research	Sac7d	N.A.	Ribosome display	SBP Info	[10], [11]
Affitin anti-PulD Sac7*40	Research	Sac7d	N.A.	Ribosome display	SBP Info	[10], [11]
Affitin anti-PulD Sac7*41	Research	Sac7d	N.A.	Ribosome display	SBP Info	[10], [11]
Affitin anti-PulD Sac7*6	Research	Sac7d	N.A.	Ribosome display	SBP Info	[10], [11]
Affitin anti-Rabbit serum albumin Sso7d\13A8	Research	Sso7d	N.A.	Phage display	SBP Info	[5]
Affitin anti-ERBB1 B10	Research	N.A.	N.A.	Ribosome display	SBP Info	[12]
Affitin anti-HEWL H4S	Research	N.A.	Escherichia coli	N.A.	SBP Info	[13]
Affitin anti-HSP110 A-C2	Research	N.A.	N.A.	Ribosome display	SBP Info	[14]
Affitin anti-HSP110 A-F12	Research	N.A.	N.A.	Ribosome display	SBP Info	[14]
Affitin anti-HSP110 A-H3	Research	N.A.	N.A.	Ribosome display	SBP Info	[14]

References

1	Potent and specific inhibition of glycosidases by small artificial binding proteins (affitins). PLoS One. 2014 May 13;9(5):e97438.
2	A novel, smaller scaffold for Affitins: Showcase with binders specific for EpCAM. Biotechnol Bioeng. 2018 Feb;115(2):290-299.
3	Tolerance of the archaeal Sac7d scaffold protein to alternative library designs: characterization of anti-immunoglobulin G Affitins. Protein Eng Des Sel. 2013 Apr;26(4):267-75.
4	Whole-bacterium ribosome display selection for isolation of highly specific anti-Staphyloccocus aureus Affitins for detection- and capture-based biomedical applications. Biotechnol Bioeng. 2019 Aug;116(8):1844-1855.
5	Phage display selection of tight specific binding variants from a hyperthermostable Sso7d scaffold protein library.FEBS J. 2016 Apr;283(7):1351-67.
6	Affinity transfer to the archaeal extremophilic Sac7d protein by insertion of a CDR. Protein Eng Des Sel. 2014 Oct;27(10):431-8.
7	Use of the Nanofitin Alternative Scaffold as a GFP-Ready Fusion Tag. PLoS One. 2015 Nov 5;10(11):e0142304.
8	Characterization of Affitin proteolytic digestion in biorelevant media and improvement of their stabilities via protein engineering. Sci Rep. 2020 Nov 12;10(1):19703.
9	Switching an anti-IgG binding site between archaeal extremophilic proteins results in Affitins with enhanced pH stability. J Biotechnol. 2014 Dec 20;192 Pt A:123-9.
10	Artificial binding proteins (Affitins) as probes for conformational changes in secretin PulD. J Mol Biol. 2008 Nov 28;383(5):1058-68.
11	Remodeling a DNA-binding protein as a specific in vivo inhibitor of bacterial secretin PulD. Proc Natl Acad Sci U S A. 2007 Nov 13;104(46):17983-8.
12	Nanofitin as a New Molecular-Imaging Agent for the Diagnosis of Epidermal Growth Factor Receptor Over-Expressing Tumors. Bioconjug Chem. 2017 Sep 20;28(9):2361-2371.
13	Reagentless fluorescent biosensors from artificial families of antigen binding proteins. Biosens Bioelectron. 2011 Jun 15;26(10):4184-90.
14	Nanofitins targeting heat shock protein 110: An innovative immunotherapeutic modality in cancer. Int J Cancer. 2021 Jun 15;148(12):3019-3031.