General Information of Synthetic Binding Protein (SBP) (ID: SBP000642)
SBP Name
Affitin anti-SasA C5
Synonyms
Affitin C5
Molecular Weight 7.8 kDa
Thermal Denaturation TEMP 77 ℃
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli
Selection Method Ribosome display
Highest Status Research
Sequence Length 68
SBP Sequence
>Affitin anti-SasA C5
GSVKVKFVVRGEEKEVDTSKIRSVWRGGKWVDFSYDDNGKQGYGYVLEKDAPKELLDMLA
RAEREKKL
3D Structure
Computationally Modelled Structure
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Template Name Sac7d
Protein Scaffold Information of This SBP
Scaffold ID PS007
Scaffold Info
[1]
Scaffold Name Affitin
Scaffold Class Non-Antibody
Fold Type One Alpha-Helix + Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Immunoglobulin G-binding protein A
BTS Info
Binder Staphylococcus aureus [ICD-11: XN6BM] Kd: 108 nM Angel University; Nantes University [1]
References
1 Whole-bacterium ribosome display selection for isolation of highly specific anti-Staphyloccocus aureus Affitins for detection- and capture-based biomedical applications. Biotechnol Bioeng. 2019 Aug;116(8):1844-1855.