General Information of Synthetic Binding Protein (SBP) (ID: SBP000649)
SBP Name
Affitin anti-GFP D8
Synonyms
Affitin D8
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli
Selection Method Ribosome display
Highest Status Research
Protein Scaffold Information of This SBP
Scaffold ID PS007
Scaffold Info
[1]
Scaffold Name Affitin
Scaffold Class Non-Antibody
Fold Type One Alpha-Helix + Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Green fluorescent protein
BTS Info
Binder Tools for cellular imaging and protein localization Kd: 2.52 nM Affilogic SAS [1]
References
1 Use of the Nanofitin Alternative Scaffold as a GFP-Ready Fusion Tag. PLoS One. 2015 Nov 5;10(11):e0142304.