General Information of Synthetic Binding Protein (SBP) (ID: SBP000645)
SBP Name
Affitin anti-HEWL M3
Synonyms
Affitin M3
Molecular Weight 7.9 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli
Highest Status Research
Sequence Length 69
SBP Sequence
>Affitin anti-HEWL M3
GSVKVKFFWNGEEKEVDTSKIVWVKRAGKSVLFIYDDNGKNGYGDVTEKDAPKEIVDMIA
RAEREKKLN
3D Structure
Computationally Modelled Structure
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Template Name Affitin H4
Protein Scaffold Information of This SBP
Scaffold ID PS007
Scaffold Info
[1]
Scaffold Name Affitin
Scaffold Class Non-Antibody
Fold Type One Alpha-Helix + Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Hen egg-white lysozyme
BTS Info
Binder Research tool Kd: 7.5 nM Angel University; Nantes University [1]
References
1 Characterization of Affitin proteolytic digestion in biorelevant media and improvement of their stabilities via protein engineering. Sci Rep. 2020 Nov 12;10(1):19703.