General Information of Synthetic Binding Protein (SBP) (ID: SBP000641)
SBP Name
Affitin anti-IgG L28K
Synonyms
Affitin L28K
Molecular Weight 7.7 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli
Selection Method Ribosome display
Highest Status Research
Sequence Length 66
SBP Sequence
>Affitin anti-IgG L28K
SVKVKFKYKGEEKEVDTSKIKKVWRDRKAAVFTYDDNGKTGYGWVFTKDAPKELLDMLAC
AEREKK
3D Structure
Computationally Modelled Structure
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Template Name Affitin D1
Protein Scaffold Information of This SBP
Scaffold ID PS007
Scaffold Info
[1]
Scaffold Name Affitin
Scaffold Class Non-Antibody
Fold Type One Alpha-Helix + Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Immunoglobulin G
BTS Info
Binder Tools for tolerating alternative library designs Kd: 31 nM Nantes University [1]
References
1 Tolerance of the archaeal Sac7d scaffold protein to alternative library designs: characterization of anti-immunoglobulin G Affitins. Protein Eng Des Sel. 2013 Apr;26(4):267-75.