General Information of Synthetic Binding Protein (SBP) (ID: SBP000631)
SBP Name
Affitin anti-CelD H3
Synonyms
Affitin H3
Molecular Weight 8.4 kDa
Thermal Denaturation TEMP 76.1 ℃
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli
Selection Method Ribosome display
Highest Status Research
PDB ID 4CJ1
Sequence Length 71
SBP Sequence
>Affitin anti-CelD H3
GSVKVKFHQIGEEKEVDTSKIKKVWRINTRLGMRVAFTYDDNGKTGRGMVPEKDAPKELL
DMLARAEGKLN
3D Structure
Experimentally Validated Structure
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Template Name Sac7d
Protein Scaffold Information of This SBP
Scaffold ID PS007
Scaffold Info
[1]
Scaffold Name Affitin
Scaffold Class Non-Antibody
Fold Type One Alpha-Helix + Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Endoglucanase D
BTS Info
Inhibitor Tools for inhibiting of glycosidases Kd: 48 nM Institut Pasteur de Montevideo; Institut Pasteur [1]
References
1 Potent and specific inhibition of glycosidases by small artificial binding proteins (affitins). PLoS One. 2014 May 13;9(5):e97438.