General Information of Synthetic Binding Protein (SBP) (ID: SBP003394)
SBP Name
Megabody anti-HEWL Mb-cAbLys3-cHopQ
Synonyms
Megabody Mb-cAbLys3-cHopQ
Molecular Weight 57.1 kDa
Thermal Denaturation TEMP 44.5 ℃
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Selection Method Yeast display
Highest Status Research
Sequence Length 539
SBP Sequence
>Megabody anti-HEWL Mb-cAbLys3-cHopQ
QVQLVESGGGLVQTKTTTSVIDTTNDAQNLLTQAQTIVNTLKDYCPILIAKSSSSNGGTN
NANTPSWQTAGGGKNSCATFGAEFSAASDMINNAQKIVQETQQLSANQPKNITQPHNLNL
NSPSSLTALAQKMLKNAQSQAEILKLANQVESDFNKLSSGHLKDYIGKCDASAISSANMT
MQNQKNNWGNGCAGVEETQSLLKTSAADFNNQTPQINQAQNLANTLIQELGNNPFRASGG
GSGGGGSGKLSDTYEQLSRLLTNDNGTNSKTSAQAINQAVNNLNERAKTLAGGTTNSPAY
QATLLALRSVLGLWNSMGYAVICGGYTKSPGENNQKDFHYTDENGNGTTINCGGSTNSNG
THSYNGTNTLKADKNVSLSIEQYEKIHEAYQILSKALKQAGLAPLNSKGEKLEAHVTTSK
YGSLRLSCAASGYTIGPYCMGWFRQAPGKEREGVAAINMGGGITYYADSVKGRFTISQDN
AKNTVYLLMNSLEPEDTAIYYCAADSTIYASYYECGHGLSTGGYGYDSWGQGTQVTVSS
3D Structure
Computationally Modelled Structure
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Template Name cAbLys3
Protein Scaffold Information of This SBP
Scaffold ID PS045
Scaffold Info
[1]
Scaffold Name Megabody
Scaffold Class Non-Antibody
Fold Type Alpha-Helices + Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Hen egg-white lysozyme
BTS Info
Binder Research tool Kd: 2.9 nM Vrije Universiteit Brussel [1]
References
1 Megabodies expand the nanobody toolkit for protein structure determination by single-particle cryo-EM. Nat Methods. 2021 Jan;18(1):60-68.