Details of the PSS

General Information of Protein Scaffold of SBP (PSS) (ID: PS045)
PSS Name
Megabody
Diagrammatic Sketch
Highest Status Research
Class Non-Antibody
Fold Type Alpha-Helices + Beta-Sheets + Loops
Thermal Denaturation TEMP 43-50℃
Sequence Length 532-539(HopQ); 899-906(YgjK)
Molecular Weight 56-101 kDa
Parental Protein of This PSS
Parental Protein Name Synonym PDB ID UniProt ID Origin
Glucosidase YgjK YgjK
3W7T
P42592
Escherichia coli (strain K12)
Helicobacter pylori adhesin domain HopQ
5LP2
Q9ZKV2
Helicobacter pylori (Campylobacter pylori)
Synthetic Binding Protein (SBP) Featuring this PSS
SBP Name Highest Status Template Expression System Selection Method Details Ref
Megabody anti-FIX Mb-Nb42-cHopQ Research Nb42 N.A. Yeast display
SBP Info
 		[1]
Megabody anti-FIX Mb-Nb42-cYgKE2 Research Nb42 N.A. Yeast display
SBP Info
 		[1]
Megabody anti-GFP Mb-Nb207-cHopQ Research Nb207 N.A. Yeast display
SBP Info
 		[1]
Megabody anti-GFP Mb-Nb207-cYgKE2 Research Nb207 N.A. Yeast display
SBP Info
 		[1]
Megabody anti-HEWL Mb-cAbLys3-cHopQ Research cAbLys3 N.A. Yeast display
SBP Info
 		[1]
Megabody anti-HEWL Mb-cAbLys3-cYgKE2 Research cAbLys3 N.A. Yeast display
SBP Info
 		[1]
Megabody anti-Sos1 Mb-Nb75-cHopQ Research Nb75 N.A. Yeast display
SBP Info
 		[1]
Megabody anti-Sos1 Mb-Nb75-cYgKE2 Research Nb75 N.A. Yeast display
SBP Info
 		[1]
References
1 Megabodies expand the nanobody toolkit for protein structure determination by single-particle cryo-EM. Nat Methods. 2021 Jan;18(1):60-68.