Details of the SBP

General Information of Synthetic Binding Protein (SBP) (ID: SBP003391)
SBP Name
Megabody anti-Sos1 Mb-Nb75-cYgKE2
Synonyms
Megabody Mb-Nb75-cYgKE2
Molecular Weight 100.2 kDa
Thermal Denaturation TEMP 43.7 ℃
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Selection Method Yeast display
Highest Status Research
Sequence Length 899
SBP Sequence
>Megabody anti-Sos1 Mb-Nb75-cYgKE2
QVQLVESGGGLVYKEETQSGLNNYARVVEKGQYDSLEIPAQVAASWESGRDDAAVFGFID
KEQLDKYVANGGKRSDWTVKFAENRSQDGTLLGYSLLQESVDQASYMYSDNHYLAEMATI
LGKPEEAKRYRQLAQQLADYINTCMFDPTTQFYYDVRIEDKPLANGCAGKPIVERGKGPE
GWSPLFNGAATQANADAVVKVMLDPKEFNTFVPLGTAALTNPAFGADIYWRGRVWVDQFW
FGLKGMERYGYRDDALKLADTFFRHAKGLTADGPIQENYNPLTGAQQGAPNFSWSAAHLY
MLYNDFFRKQASGGGSGGGGSGGGGSGNADNYKNVINRTGAPQYMKDYDYDDHQRFNPFF
DLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQDGKKVDFTLEAYSIP
GALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDGELLEKLEAKEGKPLSD
KTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEYQVHKSLPVQTEINGNRFT
SKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAFYLTASQQRWEEYLKKGLTNP
DATPEQTRVAVKAIETLNGNWRSPGGAVKFNTVTPSVTGRWFSGNQTWPWDTWKQAFAMA
HFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDLIAWNLSPERGGDGGNWNERNTKPS
LAAWSVMEVYNVTQDKTWVAEMYPKLVAYHDWWLRNRDHNGNGVPEYGATRDKAHNTESG
EMLFTVKKDSLRLSCAASGNILPINVMGWYRQTPGSQRELVATIVTSGGSTAGNTNYVDS
VKGRFTISGDNAKNTVYLQMSSLKPEDTAVYYCNLKTRRAPWATPNNYWGQGTQVTVSS
Template Name Nb75
Protein Scaffold Information of This SBP
Scaffold ID PS045
Scaffold Info
[1]
Scaffold Name Megabody
Scaffold Class Non-Antibody
Fold Type Alpha-Helices + Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Son of sevenless homolog 1
BTS Info
Binder Research tool Kd: 125.2 nM Vrije Universiteit Brussel [1]
References
1 Megabodies expand the nanobody toolkit for protein structure determination by single-particle cryo-EM. Nat Methods. 2021 Jan;18(1):60-68.