General Information of Synthetic Binding Protein (SBP) (ID: SBP000381)
SBP Name
OBody anti-HEWL AM3L09
Synonyms
OBody AM3L09
Molecular Weight 11.8 kDa
Thermal Denaturation TEMP 71.8 ℃
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli DH5 (alpha)
Selection Method Phage display
Highest Status Research
PDB ID 4GLV
Sequence Length 107
SBP Sequence
>OBody anti-HEWL AM3L09
VSPKKTHWTAEITPNLHGSEVVVAGWVAHLGDYGRVKIVKVSDREGGAAVPVYLERGKTP
DHLFKVFAELSREDVVVIKGIVEATTVTRWDTGVEIFPSEIWILNKA
3D Structure
Experimentally Validated Structure
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Template Name OBody NL8
Protein Scaffold Information of This SBP
Scaffold ID PS050
Scaffold Info
[1]
Scaffold Name OBody
Scaffold Class Non-Antibody
Fold Type Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Hen egg-white lysozyme
BTS Info
Inhibitor Tools for molecular recognition Kd: 3 nM University of Waikato; OBodies Limited [1]
References
1 Tracking molecular recognition at the atomic level with a new protein scaffold based on the OB-fold. PLoS One. 2014 Jan 20;9(1):e86050.