General Information of Synthetic Binding Protein (SBP) (ID: SBP000726)
SBP Name
VNAR anti-HEWL 4F11
Synonyms
VNAR HEL-4F11
Molecular Weight 12.0 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli
Selection Method Phage display
Highest Status Research
Sequence Length 113
SBP Sequence
>VNAR anti-HEWL 4F11
ARVDQTPRSVTKETGESLTINCVLRDASYALGSTCWYRKKSGSTNEESISKGGRYVETVN
SGSKSFSLRINDLTVEDGGTYRCGLGVAGGYCDYALCSSRYAECGDGTAVTVN
3D Structure
Computationally Modelled Structure
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Protein Scaffold Information of This SBP
Scaffold ID PS065
Scaffold Info
[1]
Scaffold Name vNAR
Scaffold Class Antibody fragment
Fold Type Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Hen egg-white lysozyme
BTS Info
Binder Research tool Kd: 20 nM University of Aberdeen [1]
References
1 Selection and characterization of naturally occurring single-domain (IgNAR) antibody fragments from immunized sharks by phage display. Mol Immunol. 2003 Sep;40(1):25-33.