General Information of Synthetic Binding Protein (SBP) (ID: SBP000880)
SBP Name
beta Roll domain anti-HEWL PN206
Synonyms
beta Roll domain-based binder PN206
Molecular Weight 16.0 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Selection Method Ribosome display
Highest Status Research
Sequence Length 152
SBP Sequence
>beta Roll domain anti-HEWL PN206
GSARDDVLIGDAGANVLRGLAGNDVLSGGAGDDWLWGDEGSDLLSGDAGNDVLCGGQGDD
TYLFGVGYGHDSIRESGGGHDTIRINAGADQLWFARQGNDLEIRILGTDDALTVHDWYRD
ADHRVEIIHAANQAVDQAGIEKLVEAMAQYPD
3D Structure
Computationally Modelled Structure
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Template Name Beta-roll
Protein Scaffold Information of This SBP
Scaffold ID PS015
Scaffold Info
[1]
Scaffold Name Beta Roll domain
Scaffold Class Non-Antibody
Fold Type (Ca2+) + Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Hen egg-white lysozyme
BTS Info
Binder Tools for controlled biomolecular recognition Kd: 6200 nM Columbia Universit [1]
References
1 Catch and Release: Engineered Allosterically Regulated -Roll Peptides Enable On/Off Biomolecular Recognition. ACS Synth Biol. 2017 Sep 15;6(9):1732-1741.