Details of the PSS

General Information of Protein Scaffold of SBP (PSS) (ID: PS039)
PSS Name	Human VH dAb
Synonyms	Human VH domain antibody
Diagrammatic Sketch
Highest Status	Research
Class	Antibody fragment
Fold Type	Beta-Sheets + Loops
Thermal Denaturation TEMP	53-82℃
Sequence Length	113-131
Molecular Weight	11-18 kDa

Parental Protein of This PSS

Parental Protein Name	Synonym		PDB ID	UniProt ID	Origin
Immunoglobulin heavy chain	VH		1FVC	P01764	Human; Synthetic construct

Synthetic Binding Protein (SBP) Featuring this PSS

SBP Name	Highest Status	Template	Expression System	Selection Method	Details	Ref
Human VH dAb anti-Alpha-amylase huVHAm416	Research	HVHP430	Escherichia coli TG1	Phage display	SBP Info	[1]
Human VH dAb anti-CD16A VH D6	Research	Human VH3C23	Escherichia coli HB2151	Phage display	SBP Info	[2], [3]
Human VH dAb anti-CD16A VH E11	Research	Human VH3C23	Escherichia coli HB2151	Phage display	SBP Info	[2], [3]
Human VH dAb anti-CEACAM6 VH428-3	Research	VH428	Escherichia coli TG1	Phage display	SBP Info	[4]
Human VH dAb anti-CEACAM6 VHB82(SS)-2	Research	VHB82(SS)	Escherichia coli TG1	Phage display	SBP Info	[4]
Human VH dAb anti-ERBB1 aEG1B4	Research	Human VH V3-23/D47	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[5]
Human VH dAb anti-ERBB1 aEG2C7	Research	Human VH V3-23/D47	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[5]
Human VH dAb anti-ERBB1 aEG2E12	Research	Human VH V3-23/D47	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[5]
Human VH dAb anti-ERBB1 aEG4D9	Research	Human V3-23/D47 VH	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[5]
Human VH dAb anti-ERBB1 aEG6B2	Research	Human V3-23/D47 VH	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[5]
Human VH dAb anti-GITR VHB82(SS)-6	Research	VHB82(SS)	Escherichia coli TG1	Phage display	SBP Info	[4]
Human VH dAb anti-HOIP clone 3	Research	N.A.	Escherichia coli	Phage display	SBP Info	[6]
Human VH dAb anti-HSA VHB82(SS)-7	Research	VHB82(SS)	Escherichia coli TG1	Phage display	SBP Info	[4]
Human VH dAb anti-MBP-intimin VH428-4	Research	VH428	Escherichia coli TG1	Phage display	SBP Info	[4]
Human VH dAb anti-MBP-intimin VHB82(SS)-8	Research	VHB82(SS)	Escherichia coli TG1	Phage display	SBP Info	[4]
Human VH dAb anti-Nitrophenace-CAP VH-N3c1	Research	VH	Escherichia coli	Phage display	SBP Info	[7]
Human VH dAb anti-OxGly VH-Ox21	Research	VH	Escherichia coli	Phage display	SBP Info	[7]
Human VH dAb anti-OxGly VH-Ox41	Research	VH	Escherichia coli	Phage display	SBP Info	[7]
Human VH dAb anti-SARS-CoV-2 A01	Research	VH-4D5	Escherichia coli	Phage display	SBP Info	[8]
Human VH dAb anti-SARS-CoV-2 ab6	Research	mCAT1	Escherichia coli HB2151	Phage display	SBP Info	[9]
Human VH dAb anti-SARS-CoV-2 ab8	Research	Human germline VH3-23	Escherichia coli HB2151	Phage display	SBP Info	[10], [3]
Human VH dAb anti-SARS-CoV-2 B01	Research	VH-4D5	Escherichia coli	Phage display	SBP Info	[8]
Human VH dAb anti-SARS-CoV-2 B02	Research	VH-4D5	Escherichia coli	Phage display	SBP Info	[8]
Human VH dAb anti-SARS-CoV-2 clone 1E2	Research	Designed sdAb	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[11]
Human VH dAb anti-SARS-CoV-2 clone 2F2	Research	Designed sdAb	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[11]
Human VH dAb anti-SARS-CoV-2 clone 3F11	Research	Designed sdAb	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[11]
Human VH dAb anti-SARS-CoV-2 clone 4D8	Research	Designed sdAb	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[11]
Human VH dAb anti-SARS-CoV-2 clone 5F8	Research	Designed sdAb	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[11]
Human VH dAb anti-SARS-CoV-2 m397	Research	mCAT1	Escherichia coli HB2151	Phage display	SBP Info	[9]
Human VH dAb anti-Acr VH E3(Y391W)	Research	Human VH domain antibody clone E3	N.A.	Discrete optimised potential energy score	SBP Info	[12]
Human VH dAb anti-Acr VH F1(M394E)	Research	Human VH domain antibody clone F1	N.A.	Discrete optimised potential energy score	SBP Info	[12]
Human VH dAb anti-HIV-1 M36	Research	VH m0	N.A.	Phage display	SBP Info	[13], [3]
Human VH dAb anti-SARS-CoV-2 n3021	Research	N.A.	Escherichia coli	Phage display	SBP Info	[14]
Human VH dAb anti-SARS-CoV-2 n3113	Research	N.A.	Escherichia coli	Phage display	SBP Info	[14]
Human VH dAb anti-SARS-CoV-2 n3130	Research	N.A.	Escherichia coli	Phage display	SBP Info	[14]
Human VH dAb anti-SARS-CoV-2 VH-Fc A01	Research	VH-4D5	N.A.	N.A.	SBP Info	[8]
Human VH dAb anti-SARS-CoV-2 VH-Fc B01	Research	VH-4D5	N.A.	N.A.	SBP Info	[8]
Human VH dAb anti-SARS-CoV-2 VH-Fc B02	Research	VH-4D5	N.A.	N.A.	SBP Info	[8]
Human VH dAb anti-SARS-CoV-2 VH2 A01	Research	VH-4D5	N.A.	N.A.	SBP Info	[8]
Human VH dAb anti-SARS-CoV-2 VH2 A01-B01	Research	VH-4D5	N.A.	N.A.	SBP Info	[8]
Human VH dAb anti-SARS-CoV-2 VH2 A01-B02	Research	VH-4D5	N.A.	N.A.	SBP Info	[8]
Human VH dAb anti-SARS-CoV-2 VH2 B01	Research	VH-4D5	N.A.	N.A.	SBP Info	[8]
Human VH dAb anti-SARS-CoV-2 VH2 B01-B02	Research	VH-4D5	N.A.	N.A.	SBP Info	[8]
Human VH dAb anti-SARS-CoV-2 VH2 B02	Research	VH-4D5	N.A.	N.A.	SBP Info	[8]
Human VH dAb anti-SARS-CoV-2 VH3 A01	Research	VH-4D5	N.A.	N.A.	SBP Info	[8]
Human VH dAb anti-SARS-CoV-2 VH3 B01	Research	VH-4D5	N.A.	N.A.	SBP Info	[8]
Human VH dAb anti-SARS-CoV-2 VH3 B02	Research	VH-4D5	N.A.	N.A.	SBP Info	[8]

References

1	Aggregation-resistant VHs selected by in vitro evolution tend to have disulfide-bonded loops and acidic isoelectric points. Protein Eng Des Sel. 2009 Feb;22(2):59-66.
2	Identification of high-affinity anti-CD16A allotype-independent human antibody domains. Exp Mol Pathol. 2016 Oct;101(2):281-289.
3	Construction of a large phage-displayed human antibody domain library with a scaffold based on a newly identified highly soluble, stable heavy chain variable domain. J Mol Biol. 2008 Oct 10;382(3):779-89.
4	Stability-Diversity Tradeoffs Impose Fundamental Constraints on Selection of Synthetic Human V H/V L Single-Domain Antibodies from In Vitro Display Libraries. Front Immunol. 2017 Dec 12;8:1759.
5	Novel single-domain antibodies against the EGFR domain III epitope exhibit the anti-tumor effect. J Transl Med. 2020 Oct 6;18(1):376.
6	Single-Domain Antibodies as Crystallization Chaperones to Enable Structure-Based Inhibitor Development for RBR E3 Ubiquitin Ligases. Cell Chem Biol. 2020 Jan 16;27(1):83-93.e9.
7	Antibody VH domains as small recognition units. Biotechnology (N Y). 1995 May;13(5):475-9.
8	Bi-paratopic and multivalent VH domains block ACE2 binding and neutralize SARS-CoV-2. Nat Chem Biol. 2021 Jan;17(1):113-121.
9	Potent neutralization of SARS-CoV-2 by human antibody heavy-chain variable domains isolated from a large library with a new stable scaffold. MAbs. 2020 Jan 1;12(1):1778435.
10	High Potency of a Bivalent Human V H Domain in SARS-CoV-2 Animal Models. Cell. 2020 Oct 15;183(2):429-441.e16.
11	Humanized single domain antibodies neutralize SARS-CoV-2 by targeting the spike receptor binding domain. Nat Commun. 2020 Sep 10;11(1):4528.
12	Optimisation of human V H domain antibodies specific to Mycobacterium tuberculosis heat shock protein (HSP16.3). J Comput Aided Mol Des. 2019 Mar;33(3):375-385.
13	Human domain antibodies to conserved sterically restricted regions on gp120 as exceptionally potent cross-reactive HIV-1 neutralizers. Proc Natl Acad Sci U S A. 2008 Nov 4;105(44):17121-6.
14	Identification of Human Single-Domain Antibodies against SARS-CoV-2. Cell Host Microbe. 2020 Jun 10;27(6):891-898.e5.