Details of the SBP | SYNBIP

General Information of Synthetic Binding Protein (SBP) (ID: SBP000960)
SBP Name	Human VH dAb anti-SARS-CoV-2 ab8
Synonyms	Human VH dAb ab8
Molecular Weight	15 kDa
Design Method	Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System	Escherichia coli HB2151
Selection Method	Phage display
Highest Status	Research
PDB ID	7MJI; 7MJH
Sequence Length	145
3D Structure
	Experimentally Validated Structure
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Template Name	Human germline VH3-23

Protein Scaffold Information of This SBP
Scaffold ID	PS039 Scaffold Info					[1] , [2]
Scaffold Name	Human VH dAb
Scaffold Class	Antibody fragment
Fold Type	Beta-Sheets + Loops

Binding Target(s) of This SBP (BTS)

BTS Name	Details	Mechanism	Application	Affinity	Research Organization	Ref
Spike glycoprotein	BTS Info	Neutralizer	SARS-CoV-2 infection (COVID-19) [ICD-11: XN109]	Kd: 19 nM	University of Pittsburgh Medical School	[1] , [2]

References

1	High Potency of a Bivalent Human V H Domain in SARS-CoV-2 Animal Models. Cell. 2020 Oct 15;183(2):429-441.e16.
2	Construction of a large phage-displayed human antibody domain library with a scaffold based on a newly identified highly soluble, stable heavy chain variable domain. J Mol Biol. 2008 Oct 10;382(3):779-89.