Details of the SBP

General Information of Synthetic Binding Protein (SBP) (ID: SBP000979)
SBP Name
Human VH dAb anti-MBP-intimin VHB82(SS)-8
Synonyms
Human VH dAb VHB82(SS)-8
Molecular Weight 13.4 kDa
Thermal Denaturation TEMP 69.3 ℃
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli TG1
Selection Method Phage display
Highest Status Research
Sequence Length 122
SBP Sequence
>Human VH dAb anti-MBP-intimin VHB82(SS)-8
QVQLQESGGGLVQPGGSLRLSCAASGFTFSRYPVAWVRQAPGKGLEWVCVISSTGTSTYY
ADSVKGRFTCSRDNSKNTLYLQMNSLRAEDTAVYYCNSQRTRLQDPNEYDYWGKGTTVTV
SS
Sequence Description Cys I and Cys II form a bridge.
3D Structure
Computationally Modelled Structure
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Template Name VHB82(SS)
Template Sequence Description Cys I and Cys II form a bridge.
Protein Scaffold Information of This SBP
Scaffold ID PS039
Scaffold Info
[1]
Scaffold Name Human VH dAb
Scaffold Class Antibody fragment
Fold Type Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Intimin
BTS Info
Binder Research tool Kd: 5230 nM National Research Council of Canada [1]
References
1 Stability-Diversity Tradeoffs Impose Fundamental Constraints on Selection of Synthetic Human V H/V L Single-Domain Antibodies from In Vitro Display Libraries. Front Immunol. 2017 Dec 12;8:1759.